Invitrogen

CD18 Monoclonal Antibody (M18/2)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 4

CD18 Antibody (MA1-10122) in Flow

Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified Monoclonal antibody (Product # MA1-10122) (concentration in sample 16 µg/mL, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension.

CD18 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD18 Antibody — Fig. 3. Inhibition of VEGF-induced vascular leakage by anti-beta2 integrin antibody. A , levels of fibronectin in the retina measured by ELISA. Anti-VEGF, VEGF plus anti-VEGF antibody ( n = 4). B , levels of beta2 integrin in the retina measured by Western blotting analysis ( n = 3). C , qualitative assessment of retinal vascular integrity demonstrated by the leakage of FITC-dextran after treatments of PBS ( control ), VEGF ( VEGF ), and VEGF plus anti-beta2 integrin antibody ( anti -beta 2 integrin ). D , quantitative analysis of relative fluorescence intensity after treatments of VEGF ( VEGF ) and VEGF plus anti-beta2 integrin antibody ( anti -beta 2 integrin ) ( n = 4). E , levels of phosphorylated FAK normalized by the total amount of FAK ( p-FAK/total FAK ) in confluent HRMECs on FN1-coated dish at 5 min after treatments of VEGF ( VEGF ) and VEGF plus anti-beta2 integrin antibody ( Anti -beta 2 integrin ) ( n = 4). F , actin cytoskeleton arrangement after treatments of PBS ( Control ), VEGF ( VEGF ), and VEGF plus anti-beta2 integrin antibody ( Anti -beta 2 integrin ) demonstrated by immunofluorescence staining of F-actin ( green ). Nucleus was identified with DAPI staining. NS , p > 0.05; *, p < 0.05; **, p < 0.01 using Kruskal-Wallis test with post hoc Dunn's multiple comparison test. {TM}

产品信息

MA1-10122

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

Assay-Dependent

流式细胞分析 (Flow)

1 µg/mL

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

M18/2

抗原

Murine cytotoxic T cell glycoproteins

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11152311

产品详细信息

This antibody recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

This antibody will not cross-react with canine.

靶标信息

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Quantitative Proteomics Reveals β2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability.

Molecular & cellular proteomics : MCP

Jo DH,Bae J,Chae S,Kim JH,Han JH,Hwang D,Lee SW,Kim JH

MA1-10122 was used in immunohistochemistry to elucidate the revelation of Beta2 integrin-mediated cytoskeletal rearrangement in vascular endothelial growth factor (VEGF)- induced retinal vascular hyperpermeability by quantitative proteomics

Sun May 01 00:00:00 EDT 2016

生物信息学

蛋白别名: CD18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; Integrin beta-2; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta

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基因别名: 2E6; AI528527; Cd18; Itgb2; LAD; LCAMB; Lfa1; MF17

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UniProt ID: (Mouse) P11835

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Entrez Gene ID: (Mouse) 16414

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功能

glycoprotein binding receptor activity protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

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