Invitrogen

CD19 Monoclonal Antibody (SJ25-C1), Alexa Fluor™ 700

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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3 篇参考文献

查看其他250个CD19抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

已发表图片 (1)
高级验证 (1)

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CD19 Antibody (MHCD1929) in Flow

Nature protocols 2010 - Published figure using CD19 monoclonal antibody (Product # MHCD1929) in Flow Cytometry

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

产品信息

MHCD1929

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SJ25-C1

抗原

Nalm 1 Human cell line

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偶联物

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

激发/发射光谱

696/719 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10373839

产品详细信息

The Alexa Fluor® 700 dye conjugate provides an excellent spectral match to the common long-wavelength excitation sources, with a high extinction coefficient.

靶标信息

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

TIL therapy broadens the tumor-reactive CD8(+) T cell compartment in melanoma patients.

Oncoimmunology

Kvistborg P,Shu CJ,Heemskerk B,Fankhauser M,Thrue CA,Toebes M,van Rooij N,Linnemann C,van Buuren MM,Urbanus JH,Beltman JB,Thor Straten P,Li YF,Robbins PF,Besser MJ,Schachter J,Kenter GG,Dudley ME,Rosenberg SA,Haanen JB,Hadrup SR,Schumacher TN

MHCD1929 was used in flow cytometry to characterize therapy-induced T cell reactivity using a panel of 45 melanoma-associated CD8(+) T cell epitopes.

Sun Jul 01 00:00:00 EDT 2012

RAC2, AEP, and ICAM1 expression are associated with CNS disease in a mouse model of pre-B childhood acute lymphoblastic leukemia.

Blood

Holland M,Castro FV,Alexander S,Smith D,Liu J,Walker M,Bitton D,Mulryan K,Ashton G,Blaylock M,Bagley S,Connolly Y,Bridgeman J,Miller C,Krishnan S,Dempsey C,Masurekar A,Stern P,Whetton A,Saha V

MHCD1929 was used in Flow cytometry/Cell sorting to suggest that combinations of processes are involved in the pathogenesis of CNS disease in pre-B-cell ALL.