Invitrogen

CD197 (CCR7) Monoclonal Antibody (4B12), APC, eBioscience™

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

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CD197 (CCR7) Antibody (17-1971-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e eFluor® 450 (Product # 48-0031-82) and 0.5 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.5 µg of Anti-Mouse CD197 (CCR7) APC (right). Viable cells in the lymphogate, as determined by staining with the 7-AAD Viability Staining Solution (Product # 00-6993-50), were used for analysis.

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产品信息

17-1971-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

4B12

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_469444

产品详细信息

Description: The 4B12 monoclonal antibody reacts with mouse CCR7, also known as EBI-1 and CD197. CCR7 is a chemokine receptor for the chemokines CCL19 (CKβ11, ELC, MIP3β, Scya19, Exodus-3) and CCL21 (CKβ9, SLC, MIP2β, Scya21, Exodus-2). In recent years, the role of chemokines in directing the migration of lymphocytes has been well-characterized. One of the most important mediators of homeostatic trafficking of naive T cells to secondary lymphoid organs (SLO) is the chemokine receptor CCR7. Binding of its ligands, CCL19 and CCL21, mediates the trans endothelial migration of T cells across high endothelial venules into SLO. It has also been demonstrated that CCR7 plays a role in the localization of dendritic cells and B cells during an immune response.

CCR7 is a chemokine receptor for the chemokines CCL19 (CKβ11, ELC, MIP3β, Scya19, Exodus-3) and CCL21 (CKβ9, SLC, MIP2β, Scya21, Exodus-2).

In addition to its significant role in the chemotaxis of lymphocytes, human CCR7 has also been recognized as a marker for a distinct subset of memory T cells, the central memory (TCM) population. These cells are characterized by the expression of CCR7 and CD62L and reside within peripheral lymphoid organs. CCR7 also plays a role in thymocyte development and its deficiency leads to disturbed thymic architecture, aberrant T cell development, and limited thymocyte expansion.

For optimal visualization of CCR7 expression on different cell types it is necessary to use multi-color staining to discriminate different cell subsets as well as following the protocol (incubation at 37C may be necessary). To address specificity, the staining profile of 4B12 has been compared to a polyclonal antibody generated against a CCR7 peptide (Bjorkdahl et al). This analysis confirms that the polyclonal antibody and 4B12 stain similar populations of cells. Furthermore, 4B12 stains mouse CCR7-GFP fusion protein-transfected RBL cells (see data in Product # 14-1971).

Applications Reported: This 4B12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 4B12 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Important: Staining with the 4B12 monoclonal antibody requires different conditions than typically used for surface-antigen staining. Please use the protocol below. Moreover, we have found that staining at 37°C, rather than 2-8°C, results in brighter 4B12 staining, as well as better resolution between positive and negative populations. Please see data for the PE 4B12 (Product # 12-1971) which demonstrates a comparison of staining at 2-8°C and 37°C. Staining with 4B12 at 37°C is not expected to interfere with co-staining other antigens, however this should be evaluated for individual experiments.

1. Prepare cell suspension as normal and block Fc gamma III R/Fc gamma II R with 5 µg/million cells purified anti-mouse CD16/32 (Product # 14-0161) for 15 minutes on ice. If red blood cell lysis is carried out as part of cell preparation, ensure that fixatives are not present in the red blood cell lysis solution as this will eliminate 4B12 staining.

2. Without washing, add 1 µg/million cells 4B12 and incubate in a 37°C waterbath or at 2-8°C (please see notes above) for 0.5 hours.

3. Wash cells 1X with 3 mL of Flow Cytometry Staining Buffer (Product # 00-4222) and decant supernatant.

4. Analyze cells on flow cytometer or proceed with secondary staining on ice as normal.

Note: Co-staining mouse CCR7 with the 4B12 antibody and the CCR7 ligand CCL19-Fc (Product # 14-1972) may be difficult due to different binding conditions required for the antibody versus the ligand, and steric hindrance which may prevent co-staining of 4B12 and CCL19-Fc. Cross-blocking experiments have demonstrated that 4B12 binding is able to prevent the detectable binding of CCL19-Fc, however not the opposite. Furthermore, the correlation between 4B12 and CCL19-Fc staining may be difficult to predict due to the presence of unknown CCL19-Fc receptors in addition to CCR7.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes.

iScience

Spath S,Roan F,Presnell SR,Höllbacher B,Ziegler SF

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Fri Sep 16 00:00:00 EDT 2022

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

17-1971 was used in Flow cytometry/Cell sorting to demonstrate cross-reactive T cell responses between SARS-CoV and SARS-CoV-2, but not MERS-CoV, in mice.

Mon Apr 05 00:00:00 EDT 2021

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

17-1971 was used in Flow cytometry/Cell sorting to demonstrate cross-reactive T cell responses between SARS-CoV and SARS-CoV-2, but not MERS-CoV, in mice.

Mon Apr 05 00:00:00 EDT 2021

Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cells.

PLoS pathogens

Lee W,Kingstad-Bakke B,Paulson B,Larsen A,Overmyer K,Marinaik CB,Dulli K,Toy R,Vogel G,Mueller KP,Tweed K,Walsh AJ,Russell J,Saha K,Reyes L,Skala MC,Sauer JD,Shayakhmetov DM,Coon J,Roy K,Suresh M

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells.

International journal of molecular medicine

Diao G,Huang J,Zheng X,Sun X,Tian M,Han J,Guo J

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions.

International journal of molecular sciences

Artinger M,Matti C,Gerken OJ,Veldkamp CT,Legler DF

17-1971-82 was used in Flow Cytometry to demonstrate that the fluorescently labeled chemokines CCL19-S6Dy649P1 and CCL21-S6Dy649P1 retain their full biological function as assessed by their abilities to mobilize intracellular calcium, to recruit β-arrestin to engaged receptors and to attract CCR7-expressing leukocytes.

Fri Apr 16 00:00:00 EDT 2021

A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions.

International journal of molecular sciences

Artinger M,Matti C,Gerken OJ,Veldkamp CT,Legler DF

Fri Apr 16 00:00:00 EDT 2021

E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions.

International journal of oncology

Huang J,Diao G,Zhang Q,Chen Y,Han J,Guo J

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Role of dendritic cell-mediated immune response in oral homeostasis: A new mechanism of osteonecrosis of the jaw.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Elsayed R,Kurago Z,Cutler CW,Arce RM,Gerber J,Celis E,Sultan H,Elashiry M,Meghil M,Sun C,Auersvald CM,Awad ME,Zeitoun R,Elsayed R,Eldin M Elshikh M,Isales C,Elsalanty ME

17-1971-82 was used in Flow Cytometry to conclude that the mechanism of Zol-induced osteonecrosis of the jaw involves disruption of DC immune functions required to clear bacterial infection and activate T cell effector response.

Sat Feb 01 00:00:00 EST 2020

Cofilin1-driven actin dynamics controls migration of thymocytes and is essential for positive selection in the thymus.

Journal of cell science

Salz A,Gurniak C,Jönsson F,Witke W

17-1971 was used in Flow cytometry/Cell sorting to describe a novel role of cofilin1 in the physiological context of migration-dependent cell differentiation.

Fri Mar 06 00:00:00 EST 2020

Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2.

BioMed research international

Zhao K,Tian Y,Wang J,Chen C,Pan B,Yan Z,Zhu S,Xu K

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Fri Sep 10 00:00:00 EDT 2021

Follicular Regulatory T Cells Can Access the Germinal Center Independently of CXCR5.

Cell reports

Vanderleyden I,Fra-Bido SC,Innocentin S,Stebegg M,Okkenhaug H,Evans-Bailey N,Pierson W,Denton AE,Linterman MA

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Tue Jan 21 00:00:00 EST 2020

MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

Zhu X,Liu H,Zhang Z,Wei R,Zhou X,Wang Z,Zhao L,Guo Q,Zhang Y,Chu C,Wang L,Li X

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Inhibition of EZH2 ameliorates bacteria-induced liver injury by repressing RUNX1 in dendritic cells.

Cell death & disease

Wang Y,Wang Q,Wang B,Gu Y,Yu H,Yang W,Ren X,Qian F,Zhao X,Xiao Y,Zhang Y,Jin M,Zhu M

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Dendritic Cells and Myeloid Derived Suppressor Cells Fully Responsive to Stimulation via Toll-Like Receptor 4 Are Rapidly Induced from Bone-Marrow Cells by Granulocyte-Macrophage Colony-Stimulating Factor.

Vaccines

Kong YY,Wilson K,Apostolopoulos V,Plebanski M

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Sat Sep 12 00:00:00 EDT 2020

Sex Differences in Mouse Popliteal Lymph Nodes.

Scientific reports

Dill-Garlow R,Chen KE,Walker AM

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Wed Jan 30 00:00:00 EST 2019

Aquaporin 4 inhibition alters chemokine receptor expression and T cell trafficking.

Scientific reports

Nicosia M,Miyairi S,Beavers A,Farr GW,McGuirk PR,Pelletier MF,Valujskikh A

17-1971-82 was used in Flow cytometry/Cell sorting to indicate that blocking AQP4 reversibly alters T lymphocyte trafficking pattern.

Wed May 15 00:00:00 EDT 2019

The Activity of the Neutral Sphingomyelinase Is Important in T Cell Recruitment and Directional Migration.

Frontiers in immunology

Collenburg L,Beyersdorf N,Wiese T,Arenz C,Saied EM,Becker-Flegler KA,Schneider-Schaulies S,Avota E

17-1971 was used in Flow cytometry/Cell sorting to support a central role of the NSM in T cell recruitment and migration both under homeostatic and inflamed conditions by regulating polarized redistribution of receptors and their coupling to the cytoskeleton.

Wed Nov 20 00:00:00 EST 2019

Ginkgolide B Protects Against Ischemic Stroke Via Modulating Microglia Polarization in Mice.

CNS neuroscience & therapeutics

Shu ZM,Shu XD,Li HQ,Sun Y,Shan H,Sun XY,Du RH,Lu M,Xiao M,Ding JH,Hu G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Anti-α-enolase antibody limits the invasion of myeloid-derived suppressor cells and attenuates their restraining effector T cell response.

Oncoimmunology

Cappello P,Tonoli E,Curto R,Giordano D,Giovarelli M,Novelli F

17-1971 was used in Flow cytometry/Cell sorting to evaluate the effects of anti-ENO1 binding on myeloid-derived suppressor cells functions and the T cell response.

Sun May 01 00:00:00 EDT 2016

Loss of Gadkin Affects Dendritic Cell Migration In Vitro.

PloS one

Schachtner H,Weimershaus M,Stache V,Plewa N,Legler DF,Höpken UE,Maritzen T

17-1971 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which Gadkin and ARP2/3 regulate dendritic cell motility and migration.

Mon Jun 20 00:00:00 EDT 2016

Retinoic acid regulates the development of a gut-homing precursor for intestinal dendritic cells.

Mucosal immunology

Zeng R,Oderup C,Yuan R,Lee M,Habtezion A,Hadeiba H,Butcher EC

17-1971 was used in Flow cytometry/Cell sorting to show that the frequency of pre-mucosal dendritic cells is reduced in vitamin A-deficient animals and in those treated with retinoic acid receptor inhibitors.

Mon Jul 01 00:00:00 EDT 2013

Canonical and noncanonical Wnt proteins program dendritic cell responses for tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Oderup C,LaJevic M,Butcher EC

17-1971 was used in Flow cytometry/Cell sorting to investigate the role of canonical and noncanonical Wnts in immune tolerance, showing that they program dendritic cell responses for tolerance.

Sat Jun 15 00:00:00 EDT 2013

CCR7-mediated LFA-1 functions in T cells are regulated by 2 independent ADAP/SKAP55 modules.

Blood

Kliche S,Worbs T,Wang X,Degen J,Patzak I,Meineke B,Togni M,Moser M,Reinhold A,Kiefer F,Freund C,Förster R,Schraven B

17-1971 was used in Immunoprecipitation to investigate mechanisms for the activation of LFA-1 and its role within the immune system.

Thu Jan 19 00:00:00 EST 2012

Migration of Th1 lymphocytes is regulated by CD152 (CTLA-4)-mediated signaling via PI3 kinase-dependent Akt activation.

PloS one

Knieke K,Lingel H,Chamaon K,Brunner-Weinzierl MC

17-1971 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms underlying initiation of Th cell migration by CD152.

Thu Aug 02 00:00:00 EDT 2012

Marginal zone macrophages suppress innate and adaptive immunity to apoptotic cells in the spleen.

Blood

McGaha TL,Chen Y,Ravishankar B,van Rooijen N,Karlsson MC

17-1971 was used in Flow cytometry/Cell sorting to support the hypothesis that MZMs are central in the clearance of apoptotic cells to minimize the immunogenicity of autoantigens.

Thu May 19 00:00:00 EDT 2011

H2 control of natural T regulatory cell frequency in the lymph node correlates with susceptibility to day 3 thymectomy-induced autoimmune disease.

Journal of immunology (Baltimore, Md. : 1950)

del Rio R,Sun Y,Alard P,Tung KS,Teuscher C

17-1971 was used in Flow cytometry/Cell sorting to confirm that quantitative trait loci on a range of chromosomes control susceptibility to autoimmune ovarian dysgenesis.

Sat Jan 01 00:00:00 EST 2011

Dendritic cells continue to capture and present antigens after maturation in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Drutman SB,Trombetta ES

17-1971 was used in Flow cytometry/Cell sorting to study the dynamics of Ag capture and presentation in situ, showing that dendritic cells continue to capture and present antigens after maturation.

Sun Aug 15 00:00:00 EDT 2010

CCR7 deficiency leads to leukocyte activation and increased clearance in response to pulmonary Pseudomonas aeruginosa infection.

Infection and immunity

Eppert BL,Motz GT,Wortham BW,Flury JL,Borchers MT

17-1971 was used in Flow cytometry/Cell sorting to investigate the roles of CCR7 in the host defense against pulmonary infection, showing that CCR7 deficiency leads to leukocyte activation and increased infection clearance.

Sat May 01 00:00:00 EDT 2010

Differential roles of migratory and resident DCs in T cell priming after mucosal or skin HSV-1 infection.

The Journal of experimental medicine

Lee HK,Zamora M,Linehan MM,Iijima N,Gonzalez D,Haberman A,Iwasaki A

17-1971 was used in Flow cytometry/Cell sorting to show that migrant and resident dendritic cells play an important role in priming CD8 and CD4 T cell responses, and their relative importance depends on the mode of infection in vivo.

Mon Feb 16 00:00:00 EST 2009

Co-immunization with an optimized plasmid-encoded immune stimulatory interleukin, high-mobility group box 1 protein, results in enhanced interferon-gamma secretion by antigen-specific CD8 T cells.

Immunology

Muthumani G,Laddy DJ,Sundaram SG,Fagone P,Shedlock DJ,Kannan S,Wu L,Chung CW,Lankaraman KM,Burns J,Muthumani K,Weiner DB

Published figure using CD197 (CCR7) monoclonal antibody (Product # 17-1971-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells.

Journal of immunology (Baltimore, Md. : 1950)

Waldner H,Sobel RA,Price N,Kuchroo VK

Mon May 01 00:00:00 EDT 2006

生物信息学

蛋白别名: C-C chemokine receptor type 7; C-C CKR-7; CD197; chemokine (C-C) receptor 7; EBI1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus-induced G-protein coupled receptor 1; MIP-3 beta receptor

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基因别名: CC-CKR-7; CCR-7; Ccr7; CD197; Cdw197; Cmkbr7; EBI1; Ebi1h

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UniProt ID: (Mouse) P47774

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Entrez Gene ID: (Mouse) 12775

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功能

signal transducer activity G-protein coupled receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

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