Invitrogen

CD20 Monoclonal Antibody (2H7), Alexa Fluor™ 532, eBioscience™

图: 1 / 16

CD20 Antibody (58-0209-42) in Flow

Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0038-42) and Mouse IgG2b kappa Isotype Control, Alexa Fluor 532 (Product # 58-4732-81) (left) or CD20 Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.

CD20 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

58-0209-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

查看12篇已发表文章 12篇已发表文章

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

2H7

偶联物

Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532

激发/发射光谱

534/553 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2815266

产品详细信息

Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2H7 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser

靶标信息

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Cancers

Care MA,Stephenson S,Owen R,Doody GM,Tooze RM

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2023

A Soluble NK-CAR Mediates the Specific Cytotoxicity of NK Cells toward the Target CD20⁺ Lymphoma Cells.

Aging and disease

Liu R,Luo Q,Luo W,Wan L,Zhu Q,Yin X,Lu X,Song Z,Wei L,Xiang Z,Zou Y

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac Disease.

Frontiers in immunology

Perez F,Ruera CN,Miculan E,Carasi P,Dubois-Camacho K,Garbi L,Guzman L,Hermoso MA,Chirdo FG

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Immunohistochemistry

Tue Jun 22 00:00:00 EDT 2021

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Genetic modification of primary human B cells to model high-grade lymphoma.

Nature communications

Caeser R,Di Re M,Krupka JA,Gao J,Lara-Chica M,Dias JML,Cooke SL,Fenner R,Usheva Z,Runge HFP,Beer PA,Eldaly H,Pak HK,Park CS,Vassiliou GS,Huntly BJP,Mupo A,Bashford-Rogers RJM,Hodson DJ

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Fri Oct 04 00:00:00 EDT 2019

Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation.

Nature communications

Colonna L,Peterson CW,Schell JB,Carlson JM,Tkachev V,Brown M,Yu A,Reddy S,Obenza WM,Nelson V,Polacino PS,Mack H,Hu SL,Zeleski K,Hoffman M,Olvera J,Furlan SN,Zheng H,Taraseviciute A,Hunt DJ,Betz K,Lane JF,Vogel K,Hotchkiss CE,Moats C,Baldessari A,Murnane RD,English C,Astley CA,Wangari S,Agricola B,Ahrens J,Iwayama N,May A,Stensland L,Huang MW,Jerome KR,Kiem HP,Kean LS

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Thu Oct 25 00:00:00 EDT 2018

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

Phase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies.

Clinical cancer research : an official journal of the American Association for Cancer Research

Bachanova V,Frankel AE,Cao Q,Lewis D,Grzywacz B,Verneris MR,Ustun C,Lazaryan A,McClune B,Warlick ED,Kantarjian H,Weisdorf DJ,Miller JS,Vallera DA

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

OMIP-030: Characterization of human T cell subsets via surface markers.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Wingender G,Kronenberg M

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Sexual Differences in Cell Loss during the Post-Hatch Development of Song Control Nuclei in the Bengalese Finch.

PloS one

Chen X,Li J,Zeng L,Zhang X,Lu X,Zuo M,Zhang X,Zeng S

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Immunohistochemistry

Mon Apr 04 00:00:00 EDT 2016

An efficient low cost method for gene transfer to T lymphocytes.

PloS one

Chicaybam L,Sodre AL,Curzio BA,Bonamino MH

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2013

Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations.

PloS one

Jensen K,Brusletto BS,Aass HC,Olstad OK,Kierulf P,Gautvik KM

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Experimental inoculation of juvenile rhesus macaques with primate enteric caliciviruses.

PloS one

Sestak K,Feely S,Fey B,Dufour J,Hargitt E,Alvarez X,Pahar B,Gregoricus N,Vinjé J,Farkas T

Published figure using CD20 monoclonal antibody (Product # 58-0209-42) in Flow Cytometry

Mon Oct 29 00:00:00 EDT 2012

生物信息学

蛋白别名: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969

查看更多收起

基因别名: B1; Bp35; CD20; CVID5; LEU-16; MS4A1; MS4A2; S7

查看更多收起

UniProt ID: (Human) P11836

查看更多收起

Entrez Gene ID: (Human) 931

查看更多收起

功能

epidermal growth factor receptor binding protein binding MHC class II protein complex binding transporter

参与通路

humoral immune response B cell proliferation