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CD200R3 Antibody (12-2001-80) in Flow
MC/9 cells were stained with Rat IgG2a kappa Isotype Control, PE (Product # 12-4321-82) (blue histogram) or CD200R3 Monoclonal Antibody, PE (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
产品信息
12-2001-80
产品规格
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
Ba13
抗原
Mouse primary basophils
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2848259
产品详细信息
Description: This Ba13 monoclonal antibody recognizes mouse CD200R3, a surface receptor expressed by basophils and mast cells.
Applications Reported: This Ba13 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This Ba13 antibody has been tested by flow cytometric analysis of MC/9 cell line. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
CD200R3 is a dimeric cell surface receptor also known as CD200RLb. CD200R3 contains two Ig-like extracellular domains and as such is a member of the immunoglobulin superfamily of receptors. The intracellular region of CD200R3 associates with the adaptor molecule DAP12, and possibly also DAP10, CD3 zeta and FcR gamma-chain, indicating that it is capable of transducing stimulatory signals. In this respect CD200R3 bears similarity to activatory receptors CD200R2 and CD200R4. In contrast, an inhibitory receptor CD200R (also known as CD200R1) relies on other adaptors. CD200R3 is primarily expressed by basophils and mast cells, cells known to be involved in anti-parasitic immune responses and frequently also allergic reactions. The surface expression of CD200R3 decreases when basophils are activated by ligating their CD16 and CD32. Remarkably, most of this downregulation is observed within the first 30 minutes after activation. Unlike CD200R, CD200R3 does not bind CD200.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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