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Invitrogen
CD218a (IL-18Ra) Monoclonal Antibody (P3TUNYA), PE-Cyanine7, eBioscience™
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CD218a (IL-18Ra) Antibody (25-5183-82) in Flow
BALB/c mouse splenocytes were stained with CD4 Monoclonal Antibody, APC (Product # 17-0042-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4321-82) (left) or 0.25 µg of CD218a (IL-18 Receptor alpha) Monoclonal Antibody, PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
25-5183-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
P3TUNYA
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2762705
产品详细信息
Description: This P3TUNYA monoclonal antibody reacts with mouse CD218a (Interleukin 18 Receptor alpha). CD218a is one of the subunits of the heterodimeric receptor complex for the IL-18 cytokine. IL-18 is a proinflammatory cytokine and member of the IL-1 family first identified by its role in IFN gamma secretion during toxic shock. Like the other members of the family, it is synthesized as an inactive precursor which requires cleavage by caspases prior to secretion of the active form. It is secreted by activated monocytes, macrophages, epithelial cells, and keratinocytes via a non-classical pathway.
IL-18 Receptor is expressed on Th1, but not naive CD4 T cells or Th2, as well as subsets of NK cells and neutrophils. The heterodimeric receptor is made up of a ligand-binding alpha chain and signal-transducing beta chain. On its own, IL-18Ra binds IL-18 with low affinity, although the affinity of this interaction increases upon forming a complex with IL-18Rb. IL-18R signaling plays a critical role in Th1 differentiation and effector function, and results in the increased expression of IFN gamma, TNF alpha, and Fas ligand from T cells and NK cells. IL-18 has also been found to synergize with IL-23 in the development and function of Th17 cells. Recently, a subset of CD8 T cells has been discovered that co-secretes IFN gamma with IL-17A and sometimes IL-22. These Tc17 cells share key transcription factors, chemokine receptors, and cytokine receptors with Th17 cells. In humans, this population has been identified by expression of CD161 and IL-18R. As a CD161 homolog has yet to be identified in mice, it has been suggested that IL-18R may be used as a surrogate for CD161 in mouse Tc17 phenotyping.
Applications Reported: This P3TUNYA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This P3TUNYA antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD218 antigen-like family member A; CD218a; IL-18R-1; IL-18R-alpha; IL-18R1; IL-1Rrp; IL1 receptor-related protein; IL1R-rp; interleukin 1 receptor related protein; Interleukin-18 receptor 1; Interleukin-18 receptor alpha
基因别名: Il18r1; Il18ralpha; Il1rrp
UniProt ID: (Mouse) Q61098
Entrez Gene ID: (Mouse) 16182
