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CD24 Antibody (M014T02Y04-A) in Flow
BALB/c mouse splenocytes were unstained (left) or stained with 0.8 µg of CD24 Monoclonal Antibody, NovaFluor Yellow 660 (right). All cells were co-stained with CD19 Monoclonal Antibody, eFluor 450 (Product # 48-0193-82). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora.
产品信息
M014T02Y04-A
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
M1/69
偶联物
激发/发射光谱
550/664 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
RRID
AB_3098994 (AB_2896809)
产品详细信息
Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: The M1/69 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.8 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow) Laser
Our internal testing shows that NovaFluor Yellow 660 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference.
靶标信息
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD24; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen
基因别名: Cd24; Cd24a; HSA; Ly-52; nectadrin
UniProt ID: (Mouse) P24807
Entrez Gene ID: (Mouse) 12484
response to hypoxia
cell activation
regulation of cytokine-mediated signaling pathway
response to molecule of bacterial origin
pre-B cell differentiation
leukocyte migration involved in inflammatory response
positive regulation of T cell mediated immune response to tumor cell
positive regulation of inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell surface receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
neuromuscular synaptic transmission
negative regulation of neuroblast proliferation
apoptotic mitochondrial changes
positive regulation of neuron projection development
cell migration
regulation of cell-cell adhesion
positive regulation of cell-cell adhesion
apoptotic nuclear changes
negative regulation of B cell proliferation
neuron projection development
T cell costimulation
chemokine receptor transport out of membrane raft
negative regulation of transforming growth factor beta3 production
positive regulation of integrin activation
positive regulation of cell adhesion mediated by integrin
regulation of cell-cell adhesion mediated by integrin
positive regulation of cell-cell adhesion mediated by integrin
negative regulation of erythrocyte clearance
homotypic cell-cell adhesion
negative regulation of erythrocyte aggregation
positive regulation of T cell homeostatic proliferation
positive regulation of activated T cell proliferation
regulation of phosphorylation
cholesterol homeostasis
positive regulation of MAP kinase activity
regulation of MAPK cascade
response to estrogen
regulation of B cell differentiation
negative regulation of neuron differentiation
respiratory burst
negative regulation of T cell homeostatic proliferation
positive regulation of alpha-beta T cell proliferation
synaptic vesicle endocytosis
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of neurogenesis
positive regulation of calcium-mediated signaling
positive regulation of protein tyrosine kinase activity
apoptotic signaling pathway
