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CD244 Antibody (35-5838-42) in Flow
Normal human peripheral blood cells were stained with CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0087-42) and Mouse IgG1 kappa Isotype Control, PE-Cyanine5.5 (Product # 35-4714-82) (left) or CD244 Monoclonal Antibody, PE-Cyanine5.5 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
35-5838-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBioC1.7 (C1.7)
偶联物
激发/发射光谱
564/692 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2784679
产品详细信息
Description: The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-gamma. Recently, it was demonstrated that binding of the C1.7 monoclonal antibody and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244.
Applications Reported: This eBioC1.7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioC1.7 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer ((Product # 00-8222-49)) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution ((Product # 00-5333-57)) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via CD244 is thought to modulate NK-cell cytolytic activity. CD244 interacts with SLAMF2, causing the activation of both SLAMF4- and SLAMF2-expressing cells. Patients with systemic lupus erythmatosus have lower than normal levels of SLAMF4 expressed on their NK cells and monocytes, suggesting that SLAMF4 may play a role in the pathology of this autoimmune disease. Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome. Alternatively spliced transcript variants encoding different isoforms of CD244 have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD244; CD244 molecule, natural killer cell receptor 2B4; h2B4; NAIL; Natural killer cell receptor 2B4; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NKR Nmrk; NKR2B4; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4
基因别名: 2B4; CD244; NAIL; NKR2B4; Nmrk; SLAMF4
UniProt ID: (Human) Q9BZW8
Entrez Gene ID: (Human) 51744
myeloid dendritic cell activation
adaptive immune response
natural killer cell activation involved in immune response
signal transduction
positive regulation of natural killer cell proliferation
innate immune response
leukocyte migration
positive regulation of inositol phosphate biosynthetic process
positive regulation of granzyme B production
positive regulation of interferon-gamma secretion
positive regulation of interleukin-8 secretion
positive regulation of CD8-positive, alpha-beta T cell proliferation
