Invitrogen

CD249 (BP-1) Monoclonal Antibody (6C3), FITC, eBioscience™

CD249 (BP-1) Antibody in Flow Cytometry (Flow)

图: 1 / 2

CD249 (BP-1) Antibody (11-5891-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) (Product # 12-0452-82) and 0.5 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (left) or 0.25 µg of Anti-Mouse BP-1 FITC (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

11-5891-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看6篇已发表文章 6篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

6C3

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465295

产品详细信息

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.

Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Embryonic keratin19⁺ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla.

Nature communications

Lucas B,White AJ,Klein F,Veiga-Villauriz C,Handel A,Bacon A,Cosway EJ,James KD,Parnell SM,Ohigashi I,Takahama Y,Jenkinson WE,Hollander GA,Lu WY,Anderson G

Published figure using CD249 (BP-1) monoclonal antibody (Product # 11-5891-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2023

Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding.

Cell stem cell

Pei W,Shang F,Wang X,Fanti AK,Greco A,Busch K,Klapproth K,Zhang Q,Quedenau C,Sauer S,Feyerabend TB,Höfer T,Rodewald HR

11-5891 was used in Flow cytometry/Cell sorting to develop the PolyloxExpress allele, enabling Cre-recombinase-dependent RNA barcoding in situ.

Thu Sep 03 00:00:00 EDT 2020

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD249 (BP-1) monoclonal antibody (Product # 11-5891-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Triplication of a 21q22 region contributes to B cell transformation through HMGN1 overexpression and loss of histone H3 Lys27 trimethylation.

Nature genetics

Lane AA,Chapuy B,Lin CY,Tivey T,Li H,Townsend EC,van Bodegom D,Day TA,Wu SC,Liu H,Yoda A,Alexe G,Schinzel AC,Sullivan TJ,Malinge S,Taylor JE,Stegmaier K,Jaffe JD,Bustin M,te Kronnie G,Izraeli S,Harris MH,Stevenson KE,Neuberg D,Silverman LB,Sallan SE,Bradner JE,Hahn WC,Crispino JD,Pellman D,Weinstock DM

11-5891 was used in Flow cytometry/Cell sorting to show that germline triplication of only 31 genes orthologous to human chromosome 21q22 confers mouse progenitor B cell self renew in vitro.

Sun Jun 01 00:00:00 EDT 2014

Deletion of genes encoding PU.1 and Spi-B in B cells impairs differentiation and induces pre-B cell acute lymphoblastic leukemia.

Blood

Sokalski KM,Li SK,Welch I,Cadieux-Pitre HA,Gruca MR,DeKoter RP

11-5891 was used in Flow cytometry/Cell sorting to investigate the impairment of differentiation of B cells and induction of pre-B cell acute lymphoblastic leukemia through the deletion of genes encoding PU.1 and Spi-1 in B cells.

Thu Sep 08 00:00:00 EDT 2011

Impaired B cell development in the absence of Krüppel-like factor 3.

Journal of immunology (Baltimore, Md. : 1950)

Vu TT,Gatto D,Turner V,Funnell AP,Mak KS,Norton LJ,Kaplan W,Cowley MJ,Agenès F,Kirberg J,Brink R,Pearson RC,Crossley M

11-5891 was used in Flow cytometry/Cell sorting to examine the function of Klf3 in B cell development by studying B lymphopoiesis in a Klf3 knockout mouse model.