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Invitrogen
CD249 (BP-1) Monoclonal Antibody (6C3), PerCP-eFluor™ 710, eBioscience™
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CD249 (BP-1) Antibody (46-5891-82) in Flow
Staining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.5 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.5 µg of Anti-Mouse BP-1 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
46-5891-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
6C3
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573797
产品详细信息
Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.
Applications Reported: This 6C3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Aminopeptidase A; aminopeptidase-A; AP-A; BP-1/6C3 antigen; BP1; CD249; EAP; Glutamyl aminopeptidase
基因别名: 6030431M22Rik; APA; Bp-1/6C3; Enpep; Ly-51; Ly51
UniProt ID: (Mouse) P16406
Entrez Gene ID: (Mouse) 13809
