Invitrogen

CD26 Monoclonal Antibody (2A6), PE, eBioscience™

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CD26 Antibody (12-0269-42) in Flow

Surface Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (left, open histogram), or Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (right, open histogram) or Anti-Human CD26 FITC (Product # 11-0269-42) (left, filled histogram), and PE (right, filled histogram). Cells in the lymphocyte population were used for analysis.

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产品信息

12-0269-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

查看9篇已发表文章 9篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2A6

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11041537

产品详细信息

Description: The 2A6 monoclonal antibody reacts with human CD26, a 110 kDa type II transmembrane dimer. CD26 is an ectoenzyme with dipeptidyl peptidase IV activity expressed by T cells and is upregulated upon T cell stimulation. It is thought that CD26 plays a role in interaction with the extracellular matrix molecules and in T cell activation.

Applications Reported: The 2A6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2A6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Association of Dipeptidylpeptidase 4 (CD26) With Chondrocyte Senescence and Radiographic Progression in Knee Osteoarthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Chen YH,Zhang X,Chou CH,Hsueh MF,Attarian D,Li YJ,Kraus VB

Published figure using CD26 monoclonal antibody (Product # 12-0269-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Association of Dipeptidylpeptidase 4 (CD26) With Chondrocyte Senescence and Radiographic Progression in Knee Osteoarthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Chen YH,Zhang X,Chou CH,Hsueh MF,Attarian D,Li YJ,Kraus VB

Published figure using CD26 monoclonal antibody (Product # 12-0269-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Synergistic roles of CBX4 chromo and SIM domains in regulating senescence of primary human osteoarthritic chondrocytes.

Arthritis research & therapy

Chen YH,Zhang X,Attarian D,Kraus VB

Published figure using CD26 monoclonal antibody (Product # 12-0269-42) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Synergistic roles of CBX4 chromo and SIM domains in regulating senescence of primary human osteoarthritic chondrocytes.

Arthritis research & therapy

Chen YH,Zhang X,Attarian D,Kraus VB

Published figure using CD26 monoclonal antibody (Product # 12-0269-42) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

CBX4 Regulates Replicative Senescence of WI-38 Fibroblasts.

Oxidative medicine and cellular longevity

Chen YH,Zhang X,Ko KY,Hsueh MF,Kraus VB

12-0269-42 was used in Flow cytometry/Cell sorting to study chromobox homolog 4 (CBX4) which has been shown to alleviate cellular senescence in human mesenchumal stem cells. CBX4 expression was investigated in relation to replicative senescence in WI-38 fibroblasts.

Thu Mar 24 00:00:00 EDT 2022

CBX4 Regulates Replicative Senescence of WI-38 Fibroblasts.

Oxidative medicine and cellular longevity

Chen YH,Zhang X,Ko KY,Hsueh MF,Kraus VB

Thu Mar 24 00:00:00 EDT 2022

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD26 monoclonal antibody (Product # 12-0269-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Dipeptidyl peptidase IV is required for endometrial carcinoma cell proliferation and tumorigenesis via the IL-6/STAT3 pathway.

The journal of obstetrics and gynaecology research

Yang X,Zhu Y,Shi Q,Zhao X,Huang Y,Yao F,Zhang Y,Wang Z

Published figure using CD26 monoclonal antibody (Product # 12-0269-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

In Vitro Comparison of the Effects of Imatinib and Ponatinib on Chronic Myeloid Leukemia Progenitor/Stem Cell Features.

Targeted oncology

Tusa I,Cheloni G,Poteti M,Silvano A,Tubita A,Lombardi Z,Gozzini A,Caporale R,Scappini B,Dello Sbarba P,Rovida E

12-0269 was used in Flow cytometry/Cell sorting to compare the effects of TKi belonging to different generations, imatinib and ponatinib (first and third generation, respectively), on progenitor/stem cell expansion potential and markers.

Thu Oct 01 00:00:00 EDT 2020

Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC.

Scientific reports

Li H,Chaitankar V,Zhu J,Chin K,Liu W,Pirooznia M,Rodgers GP

12-0269-42 was used in Flow Cytometry to indicate that OLFM4 is co-expressed with multiple stem/progenitor cell marker genes in prostate epithelial cells and acts as a novel mediator in prostate stem/progenitor cell proliferation and differentiation.

Mon Dec 14 00:00:00 EST 2020

Transcript-indexed ATAC-seq for precision immune profiling.

Nature medicine

Satpathy AT,Saligrama N,Buenrostro JD,Wei Y,Wu B,Rubin AJ,Granja JM,Lareau CA,Li R,Qi Y,Parker KR,Mumbach MR,Serratelli WS,Gennert DG,Schep AN,Corces MR,Khodadoust MS,Kim YH,Khavari PA,Greenleaf WJ,Davis MM,Chang HY

12-0269 was used in Flow cytometry/Cell sorting to combine sequencing of the TCR-encoding genes with assay for transposase-accessible chromatin with sequencing analysis at the single-cell level to provide information on the TCR specificity and epigenomic state of individual T cells.

Tue May 01 00:00:00 EDT 2018

DPPIV promotes endometrial carcinoma cell proliferation, invasion and tumorigenesis.

Oncotarget

Yang X,Zhang X,Wu R,Huang Q,Jiang Y,Qin J,Yao F,Jin G,Zhang Y

12-0269 was used in Flow cytometry/Cell sorting to investigate the role of DPPIV in endometrial carcinoma tumour progression and elucidate whether DPPIV inhibitors are potential cancer immunotherapies.

Tue Jan 31 00:00:00 EST 2017

生物信息学

蛋白别名: ADABP; ADCP-2; Adenosine deaminase complexing protein 2; CD26; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidyl-peptidase 4; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP IV; DPP-IV; T-cell activation antigen CD26; TP103

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基因别名: ADABP; ADCP2; CD26; DPP4; DPPIV; TP103

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UniProt ID: (Human) P27487

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Entrez Gene ID: (Human) 1803

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功能

virus receptor activity protease binding serine-type endopeptidase activity receptor binding protein binding serine-type peptidase activity dipeptidyl-peptidase activity identical protein binding protein homodimerization activity serine protease

参与通路