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Invitrogen
CD26 Monoclonal Antibody (M-A261), FITC, eBioscience™
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
图: 1 / 3
CD26 Antibody (BMS143FI) in Flow
Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC and CD26 FITC. As expected based on known relative expression patterns, CD26 clone M-A261 showes high levels of CD26 in a subset of lymphocytes (pink) and low level of CD26 in monocytes (orange) and granulocytes (blue).
产品信息
BMS143FI
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
M-A261
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
10 µL/Test
纯化类型
Affinity chromatography
保存液
TBS, pH 7.2-7.4, with 1% BSA
内含物
0.02% sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10597897
产品详细信息
Description: CD26 is a 110 kD type II membrane protein encoded by the DPP4 gene. It is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It also plays an integral role as a costimulatory molecule in T cell activation. CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45 and ADA.
Immunohistochemistry:
The antibody can be used to stain cryostat sections. Recommended for BMS143 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections.
Flow cytometry:
BMS143 is also suitable as primary antibody in staining for FACS analysis, BMS143FI can be used for direct staining of cells, BMS143BT can be used in a two-step procedure using the biotin-(strept-)avidin system. Recommended dilution is 1:20 as determined on A704 cells.
Immunoprecipitation:
BMS143 is also suitable for immunoprecipitation. Optimal dilutions should be determined by the individual laboratory for each application.
Applications Tested: Flow Cytometry.
Purity: >95%.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
靶标信息
CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: ADABP; ADCP-2; Adenosine deaminase complexing protein 2; CD26; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidyl-peptidase 4; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP IV; DPP-IV; T-cell activation antigen CD26; TP103
基因别名: ADABP; ADCP2; CD26; DPP4; DPPIV; TP103
UniProt ID: (Human) P27487
Entrez Gene ID: (Human) 1803
behavioral fear response
response to hypoxia
proteolysis
positive regulation of cell proliferation
negative regulation of extracellular matrix disassembly
T cell costimulation
regulation of cell-cell adhesion mediated by integrin
locomotory exploration behavior
psychomotor behavior
T cell activation
endothelial cell migration
viral entry into host cell
