Invitrogen

CD28 Recombinant Mouse Monoclonal Antibody (CD28.2), Alexa Fluor™ Plus 594

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

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CD28 Antibody (740009MP594) in Flow

CD28 expression was assessed within CD19 negative (CD19-) and CD19 positive (CD19+) subsets of human PBMCs by staining the cells with CD19 eFluor™ 450 (Product # 48-0199-42) and 0.25 µg of CD28 Recombinant Mouse Monoclonal Antibody (CD28.2), Alexa Fluor™ Plus 594 (Product # 740009MP594). Specific CD28 staining was observed in CD19- subset (pink histogram) while negligible CD28 staining was seen in the CD19+ subset (blue histogram). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Far Red Dead ... View More

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CD28 Antibody — Antibody specificity was demonstrated by relative expression. CD28 Recombinant Mouse Monoclonal Antibody (CD28.2), Alexa Fluor™ Plus 594 (Product # 7400009MP594) specifically stains the CD19 negative (CD19-) subset of human PBMCs (pink histogram) while negligible CD28 staining is seen in CD19 positive (CD19+) subset of PBMCs (blue histogram). {RE}

产品信息

740009MP594

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

CD28.2

偶联物

Alexa Fluor™ Plus 594 Alexa Fluor™ Plus 594 Alexa Fluor™ Plus 594

激发/发射光谱

590/618 nm 查看光谱

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.5

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

RRID

AB_3074187

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This CD28.2 antibody has been tested by flow cytometric analysis of human PBMCs. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 590 nm; Emission: 618 nm; Laser: Yellow Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

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