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Invitrogen
CD3 Monoclonal Antibody (4D10A6)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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CD3 Antibody (MA5-17043) in ICC/IF
Immunofluorescence analysis of CD3 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD3 Monoclonal Antibody (4D10A6) (Product # MA5-17043) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More
产品信息
MA5-17043
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
4D10A6
抗原
Purified recombinant fragment of human CD3 expressed in E. Coli.
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.03% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2538515
产品详细信息
MA5-17043 targets CD3 in indirect ELISA, ICC and IF applications and shows reactivity with Human samples.
The MA5-17043 immunogen is purified recombinant fragment of human CD3 expressed in E. Coli.
靶标信息
The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain
基因别名: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ
UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693
Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917
transmembrane signaling receptor activity
protein binding
identical protein binding
protein homodimerization activity
transcription coactivator activity
protein heterodimerization activity
receptor signaling protein activity
SH3 domain binding
protein kinase binding
receptor signaling complex scaffold activity
T cell receptor binding
defense/immunity protein
immunoglobulin receptor superfamily
viral process
T cell costimulation
Fc-gamma receptor signaling pathway involved in phagocytosis
regulation of defense response to virus by virus
regulation of immune response
T cell receptor signaling pathway
cell surface receptor signaling pathway
T cell differentiation
positive thymic T cell selection
positive regulation of transcription from RNA polymerase II promoter
positive regulation of T cell anergy
protein complex assembly
transmembrane receptor protein tyrosine kinase signaling pathway
signal complex assembly
G-protein coupled receptor signaling pathway
response to nutrient
positive regulation of gene expression
negative regulation of gene expression
positive regulation of interferon-gamma production
positive regulation of interleukin-4 production
positive regulation of T cell proliferation
T cell activation
regulation of apoptotic process
negative thymic T cell selection
positive regulation of interleukin-2 biosynthetic process
negative regulation of smoothened signaling pathway
positive regulation of alpha-beta T cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
apoptotic signaling pathway
establishment or maintenance of cell polarity
protein transport
regulation of lymphocyte apoptotic process
