Invitrogen

CD3 Monoclonal Antibody (SK7), PE-Cyanine7, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7个已发表图片

9 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

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CD3 Antibody (25-0036-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD3 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone SK7 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone SK7 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone SK7) and CD19 (clone HIB19). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used to compare CD3 staining (middle plot). Cells in the lymphocyte gate were used to compare CD3 and CD19 staining (right plot). {RE}

产品信息

25-0036-42

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Chimpanzee, Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SK7

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2637478

产品详细信息

Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast, clones OKT3 and SK7 see different epitopes.

The antibody SK7 recognizes chimpanzee CD3.

Applications Reported: This SK7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer.

The Journal of biological chemistry

Cha JH,Chan LC,Wang YN,Chu YY,Wang CH,Lee HH,Xia W,Shyu WC,Liu SP,Yao J,Chang CW,Cheng FR,Liu J,Lim SO,Hsu JL,Yang WH,Hortobagyi GN,Lin C,Yang L,Yu D,Jeng LB,Hung MC