Invitrogen

CD317 (BST2, PDCA-1) Monoclonal Antibody (26F8), Alexa Fluor™ 488, eBioscience™

CD317 (BST2, PDCA-1) Antibody in Flow Cytometry (Flow)

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CD317 (BST2, PDCA-1) Antibody (53-3179-42) in Flow

Normal human peripheral blood cells were treated with human IFN alpha 2 and then stained with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Human CD317 (BST2, PDCA-1) Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

53-3179-42

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

26F8

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1518722

产品详细信息

Description: This 26F8 monoclonal antibody reacts with human CD317 (also known as BST2 and tetherin). CD317 is a 30-36-kDa type II transmembrane protein expressed on B cells and bone marrow stromal cells. Although reports have indicated that CD317 mRNA is detectable in activated T cells, protein expression in freshly isolated primary T cells and macrophages is undetectable. Moreover, certain T cell lines, such as Jurkat, do not express detectable levels of CD317 protein. However, CD317 expression can be induced in many cell types (e.g., T and B cells, 293T, and HeLa) by IFNalpha treatment. CD317 has been associated with pre-B cell growth and the terminal differentiation of plasma B cells. More recently, this molecule has been reported to prevent HIV-1 virion release from the surface of infected cells, leading to reuptake and degradation of the virus. This activity is inhibited by the HIV-1 accessory protein Vpu. CD317 has been identified as the ligand for the ILT7 receptor.

Applications Reported: This 26F8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 26F8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein that is involved in the development and growth of B-cells. The surface expression of BST2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, NK cells and DCs (at protein level), and myeloma cells. Research studies have shown that BST2 also functions as an inhibitor of retrovirus release from human cells whose activity is antagonized by the HIV-1 accessory protein, Vpu. While BST2 causes retention of virions on cell surfaces or endocytosis into BST2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release, an activity that may represent a potential therapeutic strategy for the treatment of HIV/AIDS. Another disease associated with BST2 dysfunction is stomatitis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Japanese encephalitis virus counteracts BST2 restriction via its envelope protein E.

Virology

Li M,Wang P,Zheng Z,Hu K,Zhang M,Guan X,Fu M,Zhang D,Wang W,Xiao G,Hu Q,Liu Y

Published figure using CD317 (BST2, PDCA-1) monoclonal antibody (Product # 53-3179-42) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Nef proteins of epidemic HIV-1 group O strains antagonize human tetherin.

Cell host & microbe

Kluge SF,Mack K,Iyer SS,Pujol FM,Heigele A,Learn GH,Usmani SM,Sauter D,Joas S,Hotter D,Bibollet-Ruche F,Plenderleith LJ,Peeters M,Geyer M,Sharp PM,Fackler OT,Hahn BH,Kirchhoff F

Published figure using CD317 (BST2, PDCA-1) monoclonal antibody (Product # 53-3179-42) in Flow Cytometry

Wed Nov 12 00:00:00 EST 2014

Ig-like transcript 7, but not bone marrow stromal cell antigen 2 (also known as HM1.24, tetherin, or CD317), modulates plasmacytoid dendritic cell function in primary human blood leukocytes.

Journal of immunology (Baltimore, Md. : 1950)

Tavano B,Galao RP,Graham DR,Neil SJ,Aquino VN,Fuchs D,Boasso A

Fri Mar 15 00:00:00 EDT 2013

The expression of BST2 in human and experimental mouse brain tumors.

Experimental and molecular pathology

Wainwright DA,Balyasnikova IV,Han Y,Lesniak MS

53-3179 was used in Immunohistochemistry to investigate whether the expression of bone marrow stromal cell antigen 2 plays a critical role in brain tumour progression.