Invitrogen

CD369 (Clec7a, Dectin-1) Monoclonal Antibody (15E2), PE, eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3个已发表图片

5 篇参考文献

查看其他26个Dectin 1 (Clec7a)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD369 (Clec7a, Dectin-1) Antibody in Flow Cytometry (Flow)

图: 1 / 5

CD369 (Clec7a, Dectin-1) Antibody (12-9856-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or Anti-Human/Non-Human Primate CD369 (Clec7a, Dectin-1) PE (purple histogram). Cells in the monocyte gate were used for analysis.

Product Image Gallery

产品信息

12-9856-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Human, Non-human primate

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

15E2

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572749

产品详细信息

Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predominantly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation.

The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369.

Applications Reported: This 15E2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis.

Translational neurodegeneration

de Dios C,Abadin X,Roca-Agujetas V,Jimenez-Martinez M,Morales A,Trullas R,Mari M,Colell A

Published figure using CD369 (Clec7a, Dectin-1) monoclonal antibody (Product # 12-9856-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis.

Translational neurodegeneration

de Dios C,Abadin X,Roca-Agujetas V,Jimenez-Martinez M,Morales A,Trullas R,Mari M,Colell A

Published figure using CD369 (Clec7a, Dectin-1) monoclonal antibody (Product # 12-9856-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells.

Frontiers in cell and developmental biology

Cui D,Franz A,Fillon SA,Jannetti L,Isambert T,Fundel-Clemens K,Huber HJ,Viollet C,Ghanem A,Niwa A,Weigle B,Pflanz S

Published figure using CD369 (Clec7a, Dectin-1) monoclonal antibody (Product # 12-9856-42) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells.

Frontiers in cell and developmental biology

Cui D,Franz A,Fillon SA,Jannetti L,Isambert T,Fundel-Clemens K,Huber HJ,Viollet C,Ghanem A,Niwa A,Weigle B,Pflanz S

Published figure using CD369 (Clec7a, Dectin-1) monoclonal antibody (Product # 12-9856-42) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

IL-15 negatively regulates curdlan-induced IL-23 production by human monocyte-derived dendritic cells and subsequent Th17 response.

Northern clinics of Istanbul

Eken A,Okus Z,Erdem S,Azizoglu ZB,Haliloglu Y,Bicer A,Gur TN,Yilmaz E,Karakukcu M,Altuntas HD,Canatan H

12-9856-42 was used in Flow Cytometry to assess the effects of long- and short-term IL-15 cytokine exposure of human monocyte-derived curdlan-matured dendritic cells (DCs) on the production of Th17 cell-polarizing cytokine IL-23 and subsequent Th17 cell activation.

Thu Oct 01 00:00:00 EDT 2020

Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity.

European journal of immunology

Fischer M,Müller JP,Spies-Weisshart B,Gräfe C,Kurzai O,Hünniger K,Hochhaus A,Scholl S,Schnetzke U

Published figure using CD369 (Clec7a, Dectin-1) monoclonal antibody (Product # 12-9856-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

MiR-146a negatively regulates dectin-1-induced inflammatory responses.

Oncotarget

Du L,Chen X,Duan Z,Liu C,Zeng R,Chen Q,Li M

12-9856 was used in Flow cytometry/Cell sorting to suggest miR-146a may play the potent negative feedback regulator in inflammatory response following Dectin-1 stimulation.

Tue Jun 06 00:00:00 EDT 2017

生物信息学

蛋白别名: Beta-glucan receptor; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; CD369; DC-associated C-type lectin 1; dectin-1; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1

查看更多收起

基因别名: BGR; CANDF4; CD369; CLEC7A; CLECSF12; DECTIN1; SCARE2; UNQ539/PRO1082

查看更多收起

UniProt ID: (Human) Q9BXN2

查看更多收起

Entrez Gene ID: (Human) 64581

查看更多收起

功能

protein binding signaling pattern recognition receptor activity carbohydrate binding MHC protein binding metal ion binding

参与通路