Invitrogen

CD38 Monoclonal Antibody (HB7), PE-Cyanine7, eBioscience™

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CD38 Antibody (25-0388-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD38 PE-Cyanine7 (purple histogram). Cells in the monocyte gate were used for analysis.

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产品信息

25-0388-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HB7

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573346

产品详细信息

Description: The HB7 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is an ectoenzyme which catalyses NAD into nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR), both of which are secondary messengers. Expression of CD38 is bimodal during B cell development, modulating from high in immature cells to low in intermediate ones and back to high on mature B cells. Additionally CD38 is found in a variety of tissues and other hematopoietic cells (e.g. T cells, NK cells and monocytes) and can be used to phenotype leukemias and monitor HIV-1 progression. The CD34+CD38- population of hematopoietic stems cells is thought to define the most pluripotent cells (e.g. blast colony forming cells). In addition to surface expression, CD38 has recently been found in the nucleus where it may play a role in monitoring calcium levels.

Applications Reported: This HB7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HB7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Circulating Regulatory B-Lymphocytes in Patients with Acute Myocardial Infarction: A Pilot Study.

Journal of cardiovascular development and disease

Volodarsky I,Shimoni S,Haberman D,Mirkin V,Fabrikant Y,Yoskovich Mashriki T,Zalik A,George J

Published figure using CD38 monoclonal antibody (Product # 25-0388-42) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

Reconstructing the Lineage Histories and Differentiation Trajectories of Individual Cancer Cells in Myeloproliferative Neoplasms.

Cell stem cell

Van Egeren D,Escabi J,Nguyen M,Liu S,Reilly CR,Patel S,Kamaz B,Kalyva M,DeAngelo DJ,Galinsky I,Wadleigh M,Winer ES,Luskin MR,Stone RM,Garcia JS,Hobbs GS,Camargo FD,Michor F,Mullally A,Cortes-Ciriano I,Hormoz S

25-0388-42 was used in Flow Cytometry to investigate the consequences of the JAK2- V617F mutation on hematopoietic stem cell differentiation and self-renewal in their native microenvironment.

Thu Mar 04 00:00:00 EST 2021

Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain.

Nature chemical biology

Shukla S,Ying W,Gray F,Yao Y,Simes ML,Zhao Q,Miao H,Cho HJ,González-Alonso P,Winkler A,Lund G,Purohit T,Kim E,Zhang X,Ray JM,He S,Nikolaidis C,Ndoj J,Wang J,Jaremko Ł,Jaremko M,Ryan RJH,Guzman ML,Grembecka J,Cierpicki T

Published figure using CD38 monoclonal antibody (Product # 25-0388-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

The Master Regulator Protein BAZ2B Can Reprogram Human Hematopoietic Lineage-Committed Progenitors into a Multipotent State.

Cell reports

Arumugam K,Shin W,Schiavone V,Vlahos L,Tu X,Carnevali D,Kesner J,Paull EO,Romo N,Subramaniam P,Worley J,Tan X,Califano A,Cosma MP

25-0388 was used in Flow cytometry/Cell sorting to identify the early driving events of human B cell reprogramming.

Tue Dec 08 00:00:00 EST 2020

Genetic modification of primary human B cells to model high-grade lymphoma.

Nature communications

Caeser R,Di Re M,Krupka JA,Gao J,Lara-Chica M,Dias JML,Cooke SL,Fenner R,Usheva Z,Runge HFP,Beer PA,Eldaly H,Pak HK,Park CS,Vassiliou GS,Huntly BJP,Mupo A,Bashford-Rogers RJM,Hodson DJ

Published figure using CD38 monoclonal antibody (Product # 25-0388-42) in Flow Cytometry

Fri Oct 04 00:00:00 EDT 2019

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

25-0388 was used in Flow cytometry/Cell sorting to reveal an unprecedented presence of multi-Ig specificity in some single B cells, implying regulation of Ig gene rearrangement and class switching that differs from the classical mechanisms of both the "one cell - one antibody" rule and CSR.

Fri Oct 20 00:00:00 EDT 2023

生物信息学

蛋白别名: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10

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基因别名: ADPRC 1; ADPRC1; CD38

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UniProt ID: (Human) P28907

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Entrez Gene ID: (Human) 952

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功能

NAD+ nucleosidase activity transferase activity phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

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