Invitrogen

CD44 Monoclonal Antibody (IM7)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4个已发表图片

32 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 7

CD44 Antibody (MA1-10225) in WB

Western blotting analysis of CD44 in HeLa cells (positive; lane 2) and MOLT-4 cells (negative; lane 1) using anti-CD44 (IM7) purified Monoclonal antibody (Product # MA1-10225); non-reducing conditions.

CD44 Antibody in Immunohistochemistry (IHC)

CD44 Antibody in Western Blot, Flow Cytometry (WB, Flow)

Product Image Gallery

CD44 Antibody — S1 Fig Analysis of CD44 expression in Wild type (WT) and CD44-deficient NOD mice. A) PCR analysis of tail DNA discriminates between WT (#1, 120bp product band), CD44-deficient (#2, 300bp product band) and heterozygous (#3, both bands) mice. (-) PCR negative control including all the PCR compounds except DNA. B) Western blot analysis of spleen cell extract subjected to 10% SDS-PAGE followed by immunoblotting with anti-CD44 antibody and C) flow cytometry analysis with 0.5 µg/mL IM7.8.1 FITC-conjugated anti-CD44 mAb (eBioscience, see [16]) reveal CD44 signal in WT, but not in CD44-deficient splenocytes. IgG2b, Isotype-matched control antibody. {KO}

产品信息

MA1-10225

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

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流式细胞分析 (Flow)

1 µg/mL

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免疫沉淀 (IP)

Assay-dependent

阻断反应 (BLOCK)

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其他PubMed (Misc)

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产品规格

种属反应

Dog, Horse, Cat, Human, Mouse

已发表种属

Dog, Human, Mouse, Pig

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

IM7

抗原

Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11154299

产品详细信息

This antibody reacts with all isoforms of mouse CD44.

Western Blot: non-reducing conditions.

靶标信息

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints.

Stem cell research & therapy

Teunissen M,Ahrens NS,Snel L,Narcisi R,Kamali SA,van Osch GJVM,Meij BP,Mastbergen SC,Sivasubramaniyan K,Tryfonidou MA

MA1-10225 was used in Immunohistochemistry to show that OA cSM-MPCs may be a less optimal candidate for the cell-based treatment of OA than normal cSM-MPCs.

Mon Sep 05 00:00:00 EDT 2022

Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints.

Stem cell research & therapy

Teunissen M,Ahrens NS,Snel L,Narcisi R,Kamali SA,van Osch GJVM,Meij BP,Mastbergen SC,Sivasubramaniyan K,Tryfonidou MA

MA1-10225 was used in Immunohistochemistry to show that OA cSM-MPCs may be a less optimal candidate for the cell-based treatment of OA than normal cSM-MPCs.

Mon Sep 05 00:00:00 EDT 2022

SingleCellSignalR: inference of intercellular networks from single-cell transcriptomics.

Nucleic acids research

Cabello-Aguilar S,Alame M,Kon-Sun-Tack F,Fau C,Lacroix M,Colinge J

MA1-10225 was used in Immunohistochemistry-immunofluorescence to demonstrate SingleCellSignalR on mouse epidermis data and discover an oriented communication structure from external to basal layers.

Thu Jun 04 00:00:00 EDT 2020

SingleCellSignalR: inference of intercellular networks from single-cell transcriptomics.

Nucleic acids research

Cabello-Aguilar S,Alame M,Kon-Sun-Tack F,Fau C,Lacroix M,Colinge J

MA1-10225 was used in Immunohistochemistry-immunofluorescence to demonstrate SingleCellSignalR on mouse epidermis data and discover an oriented communication structure from external to basal layers.

Thu Jun 04 00:00:00 EDT 2020

xCT inhibition sensitizes tumors to γ-radiation via glutathione reduction.

Oncotarget

Cobler L,Zhang H,Suri P,Park C,Timmerman LA

MA1-10225 was used in Immunohistochemistry to demonstrate that targeted inhibition of xCT provides tumour-specific sensitisation to radiation therapy.

Fri Aug 17 00:00:00 EDT 2018

Hyaluronan content governs tissue stiffness in pancreatic islet inflammation.

The Journal of biological chemistry

Nagy N,de la Zerda A,Kaber G,Johnson PY,Hu KH,Kratochvil MJ,Yadava K,Zhao W,Cui Y,Navarro G,Annes JP,Wight TN,Heilshorn SC,Bollyky PL,Butte MJ

MA1-10225 was used in Immunohistochemistry to study the role of hyaluronan in the regulation of the mechanical properties of pancreatic islets.

Fri Jan 12 00:00:00 EST 2018

Lung Pathologies in a Chronic Inflammation Mouse Model Are Independent of Eosinophil Degranulation.

American journal of respiratory and critical care medicine

Jacobsen EA,Ochkur SI,Doyle AD,LeSuer WE,Li W,Protheroe CA,Colbert D,Zellner KR,Shen HH,Irvin CG,Lee JJ,Lee NA

MA1-10225 was used in flow cytometry to discuss differences in eosinophil degranulation between humans and mice

Mon May 15 00:00:00 EDT 2017

Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice.

Cellular and molecular life sciences : CMLS

van Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston A

MA110225 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis

Thu Jun 01 00:00:00 EDT 2017

Deficiency of N-myristoylation reveals calcineurin activity as regulator of IFN-γ-producing γδ T cells.

Journal of leukocyte biology

Rampoldi F,Brunk F,Bonrouhi M,Federico G,Krunic D,Porubsky S,Gröne HJ,Popovic ZV

MA110225 was used in flow cytometry to evaluate the potential role of the attachment of myristic acid to the N-terminal glycine of proteins on the activation of gamma delta T cells

Sat Apr 01 00:00:00 EDT 2017

NKG2C/E Marks the Unique Cytotoxic CD4 T Cell Subset, ThCTL, Generated by Influenza Infection.

Journal of immunology (Baltimore, Md. : 1950)

Marshall NB,Vong AM,Devarajan P,Brauner MD,Kuang Y,Nayar R,Schutten EA,Castonguay CH,Berg LJ,Nutt SL,Swain SL

MA110225 was used in flow cytometry to determine that NKG2C/E identifies the CD4 T cell effector subset ThCTL that develop in the lung during influenza A virus infection in mice

Wed Feb 01 00:00:00 EST 2017

Soluble CD83 Inhibits T Cell Activation by Binding to the TLR4/MD-2 Complex on CD14⁺ Monocytes.

Journal of immunology (Baltimore, Md. : 1950)

Horvatinovich JM,Grogan EW,Norris M,Steinkasserer A,Lemos H,Mellor AL,Tcherepanova IY,Nicolette CA,DeBenedette MA

MA110225 was used in blocking or activating experiment to implicate myeloid differentiation factor-2 as the high-affinity soluble CD83 binding partner

Wed Mar 15 00:00:00 EDT 2017

ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content.

Scientific reports

Nowyhed HN,Chandra S,Kiosses W,Marcovecchio P,Andary F,Zhao M,Fitzgerald ML,Kronenberg M,Hedrick CC

MA110225 was used in flow cytometry to show that ABCA7 regulates natural killer T cell development in a cell-extrinsic manner

Mon Jan 16 00:00:00 EST 2017

Collaborative Interferon-γ and Interleukin-17 Signaling Protects the Oral Mucosa from Staphylococcus aureus.

The American journal of pathology

Barin JG,Talor MV,Schaub JA,Diny NL,Hou X,Hoyer M,Archer NK,Gebremariam ES,Davis MF,Miller LS,Rose NR,Čiháková D

MA110225 was used in flow cytometry to study how IL-17 and IFN-gamma control Staphylococcus aureus infection

Thu Sep 01 00:00:00 EDT 2016

lck-Driven Cre Expression Alters T Cell Development in the Thymus and the Frequencies and Functions of Peripheral T Cell Subsets.

Journal of immunology (Baltimore, Md. : 1950)

Carow B,Gao Y,Coquet J,Reilly M,Rottenberg ME

MA110225 was used in flow cytometry to implement lck-cre transgenic mice to study the role of loxP-targeted genes in T cell development and function

Thu Sep 15 00:00:00 EDT 2016

The autophagy gene Atg16l1 differentially regulates Treg and TH2 cells to control intestinal inflammation.

eLife

Kabat AM,Harrison OJ,Riffelmacher T,Moghaddam AE,Pearson CF,Laing A,Abeler-Dörner L,Forman SP,Grencis RK,Sattentau Q,Simon AK,Pott J,Maloy KJ

MA1-10225 was used in flow cytometry to establish that autophagy is essential for maintenance of a balanced CD4 positive intestinal T cell response

Wed Feb 24 00:00:00 EST 2016

Glibenclamide Prevents Diabetes in NOD Mice.

PloS one

Lamprianou S,Gysemans C,Bou Saab J,Pontes H,Mathieu C,Meda P

MA110225 was used in flow cytometry to explore if the anti-diabetic sulphonylurea glibenclamide protects insulin-producing cells against conditions mimicking those expected at the onset of type 1 diabetes

Tue Jul 18 00:00:00 EDT 2017

Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells.

Stem cell research & therapy

Valle YL,Almalki SG,Agrawal DK

MA110225 was used in flow cytometry and immunocytochemistry to assess the presence of vitamin D machinery on porcine adipose-derived MSCs

Wed Aug 17 00:00:00 EDT 2016

GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal.

Cell death & disease

Le Q,Yao W,Chen Y,Yan B,Liu C,Yuan M,Zhou Y,Ma L

MA110225 was used in flow cytometry to use knockout mice to determine if GRK6 contributes to hematopoiesis

Thu Nov 24 00:00:00 EST 2016

Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses.

Nature immunology

Shrestha S,Yang K,Guy C,Vogel P,Neale G,Chi H

MA1-10225 was used in flow cytometry to show that the PTEN-mTORC2 axis maintains T regulatory cell stability and coordinates their control of effector responses

Sun Feb 01 00:00:00 EST 2015

IL-25-responsive, lineage-negative KLRG1(hi) cells are multipotential 'inflammatory' type 2 innate lymphoid cells.

Nature immunology

Huang Y,Guo L,Qiu J,Chen X,Hu-Li J,Siebenlist U,Williamson PR,Urban JF,Paul WE

MA1-10225 was used in Flow Cytometry to characterise the existence of an inflammatory ILC2 population responsive to interleukin 25 that complements IL-33-responsive natural ILC2 cells.

Sun Feb 01 00:00:00 EST 2015

ICOS and Bcl6-dependent pathways maintain a CD4 T cell population with memory-like properties during tuberculosis.

The Journal of experimental medicine

Moguche AO,Shafiani S,Clemons C,Larson RP,Dinh C,Higdon LE,Cambier CJ,Sissons JR,Gallegos AM,Fink PJ,Urdahl KB

MA1-10225 was used in flow cytometry to assess a CD 4 T-cell population during tuberculosis that has memory-like properties maintained by Bcl6 and ICOS-dependent pathways

Mon May 04 00:00:00 EDT 2015

Rpl22 Loss Impairs the Development of B Lymphocytes by Activating a p53-Dependent Checkpoint.

Journal of immunology (Baltimore, Md. : 1950)

Fahl SP,Harris B,Coffey F,Wiest DL

MA1-10225 was used in flow cytometry to investigate the role of Rpl22 during early B cell development

Thu Jan 01 00:00:00 EST 2015

Can CD44 Be a Mediator of Cell Destruction? The Challenge of Type 1 Diabetes.

PloS one

Assayag-Asherie N,Sever D,Bogdani M,Johnson P,Weiss T,Ginzberg A,Perles S,Weiss L,Sebban LE,Turley EA,Okon E,Raz I,Naor D

MA1-10225 was used in immunohistochemistry to investigate how CD44 modulation induced resistance to type 1 diabetes in NOD mice

Thu Jun 23 00:00:00 EDT 2016

Can CD44 Be a Mediator of Cell Destruction? The Challenge of Type 1 Diabetes.

PloS one

Assayag-Asherie N,Sever D,Bogdani M,Johnson P,Weiss T,Ginzberg A,Perles S,Weiss L,Sebban LE,Turley EA,Okon E,Raz I,Naor D

MA1-10225 was used in immunohistochemistry to investigate how CD44 modulation induced resistance to type 1 diabetes in NOD mice

Thu Jun 23 00:00:00 EDT 2016

Can CD44 Be a Mediator of Cell Destruction? The Challenge of Type 1 Diabetes.

PloS one

Assayag-Asherie N,Sever D,Bogdani M,Johnson P,Weiss T,Ginzberg A,Perles S,Weiss L,Sebban LE,Turley EA,Okon E,Raz I,Naor D

MA1-10225 was used in immunohistochemistry to investigate how CD44 modulation induced resistance to type 1 diabetes in NOD mice

Thu Jun 23 00:00:00 EDT 2016

Can CD44 Be a Mediator of Cell Destruction? The Challenge of Type 1 Diabetes.

PloS one

Assayag-Asherie N,Sever D,Bogdani M,Johnson P,Weiss T,Ginzberg A,Perles S,Weiss L,Sebban LE,Turley EA,Okon E,Raz I,Naor D

MA1-10225 was used in immunohistochemistry to investigate how CD44 modulation induced resistance to type 1 diabetes in NOD mice

Thu Jun 23 00:00:00 EDT 2016

Interferon-γ induces expression of MHC class II on intestinal epithelial cells and protects mice from colitis.

PloS one

Thelemann C,Eren RO,Coutaz M,Brasseit J,Bouzourene H,Rosa M,Duval A,Lavanchy C,Mack V,Mueller C,Reith W,Acha-Orbea H

MA1-10225 was used in Flow Cytometry to investigate how epithelial MHCII expression is induced and how a deficiency in inducible epithelial MHCII expression alters susceptibility to colitis and the outcome of colon-specific immune responses.

Mon Dec 22 00:00:00 EST 2014

CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.

Journal of immunology (Baltimore, Md. : 1950)

Wensveen FM,Unger PP,Kragten NA,Derks IA,ten Brinke A,Arens R,van Lier RA,Eldering E,van Gisbergen KP

MA1-10225 was used in Flow Cytometry to investigate how BIm-dependent apoptosis is initiated in CD8 T cells.

Tue May 01 00:00:00 EDT 2012

c-Myb is required for pro-B cell differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Fahl SP,Crittenden RB,Allman D,Bender TP

MA1-10225 was used in flow cytometry to determine the roles of c-Myb during lymphocyte development

Sun Nov 01 00:00:00 EDT 2009

Enhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines.

Blood

Ahonen CL,Wasiuk A,Fuse S,Turk MJ,Ernstoff MS,Suriawinata AA,Gorham JD,Kedl RM,Usherwood EJ,Noelle RJ

MA1-10225 was used in Immunocytochemistry to report the therapeutic effects of combinatorial use of CD40 and TLR agonists as a cancer vaccine.

Sat Mar 15 00:00:00 EDT 2008

Enhanced antigen-specific primary CD4+ and CD8+ responses by codelivery of ovalbumin and toll-like receptor ligand monophosphoryl lipid A in poly(D,L-lactic-co-glycolic acid) nanoparticles.

Journal of biomedical materials research. Part A

Hamdy S,Elamanchili P,Alshamsan A,Molavi O,Satou T,Samuel J

MA1-10225 was used in flow cytometry to evaluate the use of biodegradable poly(D,L-lactic-co-glycolic acid) nanoparticles as a vaccine delivery system

Fri Jun 01 00:00:00 EDT 2007

Continuous activation of autoreactive CD4+ CD25+ regulatory T cells in the steady state.

The Journal of experimental medicine

Fisson S,Darrasse-Jèze G,Litvinova E,Septier F,Klatzmann D,Liblau R,Salomon BL

MA1-10225 was used in flow cytometry to report two Treg subsets with distinct phenotypes and homeostasis in normal unmanipulated mice.

Mon Sep 01 00:00:00 EDT 2003

CD8+ tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ.

Journal of immunology (Baltimore, Md. : 1950)

Radoja S,Saio M,Frey AB

MA1-10225 was used in flow cytometry to characterize murine tumor-infiltrating lymphocytes.

Tue May 15 00:00:00 EDT 2001

Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.

The Journal of experimental medicine

Lesley J,He Q,Miyake K,Hamann A,Hyman R,Kincade PW

MA1-10225 was used in flow cytometry to study CD44 and hyaluronic acid interactions

Wed Jan 01 00:00:00 EST 1992

生物信息学

蛋白别名: CD44; CD44 antigen; CD44 antigen-like protein; CD44 molecule; CD44 molecule (Indian blood group); CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; extracellular matrix receptor-III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; lymphocyte surface antigen precursor CD44; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44

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基因别名: AU023126; AW121933; AW146109; CD44; CDW44; CSPG8; ECMR-III; HCELL; HERMES; HUTCH-I; IN; LHR; Ly-24; MC56; MDU2; MDU3; MIC4; Pgp-1; Pgp1

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UniProt ID: (Human) Q16064, (Mouse) P15379

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Entrez Gene ID: (Human) 960, (Horse) 100034221, (Dog) 403939, (Mouse) 12505

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功能

cytokine receptor activity protein binding collagen binding hyaluronic acid binding hyalurononglucosaminidase activity epidermal growth factor receptor binding protein kinase binding cytokine binding phosphoprotein binding transmembrane signal receptor

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