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Proteintech

CD45 Monoclonal Antibody (4E9B2)

28 篇参考文献

查看其他310个CD45抗体

说明书

实验方案

技术支持

货号 60287-1-IG

此产品在中国无法购买

150 µL

说明书

实验方案

技术支持

抗体检测数据 (13)

Application

CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 13

CD45 Antibody (60287-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using (Product # 60287-1-Ig) CD45 antibody at dilution of 1:8,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD45 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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产品信息

60287-1-IG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:5,000-1:20,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:100

免疫细胞化学 (ICC/IF)

查看12篇已发表文章 12篇已发表文章

流式细胞分析 (Flow)

0.2 µg/1x10^6 cells

查看6篇已发表文章 6篇已发表文章

不适用 (N/A)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Pig, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

4E9B2

抗原

CD45 Fusion Protein Ag13712 (550-750 aa encoded by BC014239)

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1.67 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: KIYDLHKKR SCNLDEQQEL VERDDEKQLM NVEPIHADIL LETYKRKIAD EGRLFLAEFQ SIPRVFSKFP IKEARKPFNQ NKNRYVDILP YDYNRVELSE INGDAGSNYI NASYIDGFKE PRKYIAAQGP RDETVDDFWR MIWEQKATVI VMVTRCEEGN RNKCAEYWPS MEEGTRAFGD VVVKINQHKR CPDYIIQKLN IV (550-750 aa encoded by BC014239)

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; RT7; T200

UniProt ID: (Human) P08575, (Mouse) P06800, (Rat) P04157

Entrez Gene ID: (Human) 5788, (Pig) 100522631, (Mouse) 19264, (Rat) 24699

功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein kinase regulator activity protein phosphatase protein modifying enzyme extracellular matrix protein transmembrane signal receptor

参与通路

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway

CD45 Monoclonal Antibody (4E9B2)

CD45 Antibody (60287-1-IG) in IHC (P)

产品信息

60287-1-IG

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

不适用 (N/A)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

产品详细信息

靶标信息

生物信息学

性能保障

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