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Invitrogen

CD45 Monoclonal Antibody (YAML501.4)

6个已发表图片

12 篇参考文献

查看其他310个CD45抗体

说明书

实验方案

技术支持

货号 MA5-17687

CNY 5,262.00

200 µg

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说明书

实验方案

技术支持

抗体检测数据 (3)
已发表图片 (6)

Application

CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 9

CD45 Antibody (MA5-17687) in IHC (P)

Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification with DAPI counterstain. Primary antibody used was a CD45/PTPRC monoclonal antibody (clone YAML501.4) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-rat at a 1:500 dilution and Normal Goat Serum (blocking)

CD45 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD45 Antibody in Immunocytochemistry (ICC/IF)

CD45 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

MA5-17687

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:500

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

0.5 µg/1x10^6 cells

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

YAML501.4

抗原

Rat monoclonal antibody raised against acute myeloid leukemia cells of human origin.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539077

产品详细信息

MA5-17687 targets CD45/PTPRC in FACS and WB applications and shows reactivity with Human samples.

The MA5-17687 immunogen is rat monoclonal antibody raised against acute myeloid leukemia cells of human origin.

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Safety Profiling of Tumor-targeted T Cell-Bispecific Antibodies with Alveolus Lung- and Colon-on-Chip.

Bio-protocol

Kerns JS,Belgur C,Kanellias M,Manatakis DV,Barrile R,Tien-Street W,Ewart L,Gjorevski N,Cabon L

MA5-17687 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to evaluate the safety profile of T cell-bispecific antibodies (TCBs) targeting tumor antigens.

Thu Jan 05 00:00:00 UTC 2023

Expression of antioxidant enzymes in lesions of multiple sclerosis and its models.

Scientific reports

Moezzi D,Dong Y,Jain RW,Lozinski BM,Ghorbani S,D'Mello C,Wee Yong V

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunohistochemistry

Tue Jul 26 00:00:00 UTC 2022

Expression of antioxidant enzymes in lesions of multiple sclerosis and its models.

Scientific reports

Moezzi D,Dong Y,Jain RW,Lozinski BM,Ghorbani S,D'Mello C,Wee Yong V

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunohistochemistry

Tue Jul 26 00:00:00 UTC 2022

Phenotypic and Histological Distribution Analysis Identify Mast Cell Heterogeneity in Non-Small Cell Lung Cancer.

Cancers

Leveque E,Rouch A,Syrykh C,Mazières J,Brouchet L,Valitutti S,Espinosa E,Lafouresse F

MA5-17687 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to reveal that TAMCs constitute a heterogeneous population and indicates that MC subsets should be considered for patients' stratification and management in future research.

Wed Mar 09 00:00:00 UTC 2022

Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination.

Nature communications

Ghorbani S,Jelinek E,Jain R,Buehner B,Li C,Lozinski BM,Sarkar S,Kaushik DK,Dong Y,Wight TN,Karimi-Abdolrezaee S,Schenk GJ,Strijbis EM,Geurts J,Zhang P,Ling CC,Yong VW

MA5-17687 was used in Immunohistochemistry to explore high versican-V1 expression in multiple sclerosis (MS) lesions with deficient premyelinating oligodendrocytes, and highlight its selective upregulation amongst CSPG members in experimental autoimmune encephalomyelitis (EAE) lesions modeling MS.

Wed May 04 00:00:00 UTC 2022

Integration of Functional Imaging, Cytometry, and Unbiased Proteomics Reveals New Features of Endothelial-to-Mesenchymal Transition in Ischemic Mitral Valve Regurgitation in Human Patients.

Frontiers in cardiovascular medicine

Lupieri A,Nagata Y,Passos LSA,Beker-Greene D,Kirkwood KA,Wylie-Sears J,Alvandi Z,Higashi H,Hung JW,Singh SA,Bischoff J,Levine RA,Aikawa E

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunohistochemistry

Wed Apr 20 00:00:00 UTC 2022

Integration of Functional Imaging, Cytometry, and Unbiased Proteomics Reveals New Features of Endothelial-to-Mesenchymal Transition in Ischemic Mitral Valve Regurgitation in Human Patients.

Frontiers in cardiovascular medicine

Lupieri A,Nagata Y,Passos LSA,Beker-Greene D,Kirkwood KA,Wylie-Sears J,Alvandi Z,Higashi H,Hung JW,Singh SA,Bischoff J,Levine RA,Aikawa E

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunohistochemistry

Wed Apr 20 00:00:00 UTC 2022

Chronic mTOR activation induces a degradative smooth muscle cell phenotype.

The Journal of clinical investigation

Li G,Wang M,Caulk AW,Cilfone NA,Gujja S,Qin L,Chen PY,Chen Z,Yousef S,Jiao Y,He C,Jiang B,Korneva A,Bersi MR,Wang G,Liu X,Mehta S,Geirsson A,Gulcher JR,Chittenden TW,Simons M,Humphrey JD,Tellides G

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunohistochemistry

Mon Mar 02 00:00:00 UTC 2020

Chronic mTOR activation induces a degradative smooth muscle cell phenotype.

The Journal of clinical investigation

Li G,Wang M,Caulk AW,Cilfone NA,Gujja S,Qin L,Chen PY,Chen Z,Yousef S,Jiao Y,He C,Jiang B,Korneva A,Bersi MR,Wang G,Liu X,Mehta S,Geirsson A,Gulcher JR,Chittenden TW,Simons M,Humphrey JD,Tellides G

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunohistochemistry

Mon Mar 02 00:00:00 UTC 2020

Evolution of Estrogen Receptor Status from Primary Tumors to Metastasis and Serially Collected Circulating Tumor Cells.

International journal of molecular sciences

Forsare C,Bendahl PO,Moberg E,Levin Tykjær Jørgensen C,Jansson S,Larsson AM,Aaltonen K,Rydén L

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunocytochemistry

Mon Apr 20 00:00:00 UTC 2020

Evolution of Estrogen Receptor Status from Primary Tumors to Metastasis and Serially Collected Circulating Tumor Cells.

International journal of molecular sciences

Forsare C,Bendahl PO,Moberg E,Levin Tykjær Jørgensen C,Jansson S,Larsson AM,Aaltonen K,Rydén L

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunocytochemistry

Mon Apr 20 00:00:00 UTC 2020

Evolution of Estrogen Receptor Status from Primary Tumors to Metastasis and Serially Collected Circulating Tumor Cells.

International journal of molecular sciences

Forsare C,Bendahl PO,Moberg E,Levin Tykjær Jørgensen C,Jansson S,Larsson AM,Aaltonen K,Rydén L

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunocytochemistry

Mon Apr 20 00:00:00 UTC 2020

The tyrosine phosphatase SHP2 controls TGFβ-induced STAT3 signaling to regulate fibroblast activation and fibrosis.

Nature communications

Zehender A,Huang J,Györfi AH,Matei AE,Trinh-Minh T,Xu X,Li YN,Chen CW,Lin J,Dees C,Beyer C,Gelse K,Zhang ZY,Bergmann C,Ramming A,Birchmeier W,Distler O,Schett G,Distler JHW

Published figure using CD45 monoclonal antibody (Product # MA5-17687) in Immunofluorescence

Tue Aug 14 00:00:00 UTC 2018

Filtration-based enrichment of circulating tumor cells from all prostate cancer risk groups.

Urologic oncology

Awe JA,Saranchuk J,Drachenberg D,Mai S

MA5-17687 was used in Immunohistochemistry to present an effective filtration-based isolation method for prostate cancer circulating tumor cells in all risk groups, thus providing a reliable platform for the study of individual prostate cancer cases.

Mon May 01 00:00:00 UTC 2017

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

UniProt ID: (Human) P08575

Entrez Gene ID: (Human) 5788

功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

参与通路

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

CD45 Monoclonal Antibody (YAML501.4)

CD45 Antibody (MA5-17687) in IHC (P)

产品信息

MA5-17687

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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CD45 Monoclonal Antibody (YAML501.4)

CD45 Antibody (MA5-17687) in IHC (P)

产品信息

MA5-17687

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID