Proteintech

CD47 Polyclonal Antibody

CD47 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD47 Antibody (20305-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (Product # 20305-1-AP) (CD47 antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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产品信息

20305-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看9篇已发表文章 9篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫细胞化学 (ICC/IF)

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不适用 (N/A)

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

CD47 Fusion Protein Ag14132 (1-150 aa encoded by BC010016)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: MWPLVAALL LGSACCGSAQ LLFNKTKSVE FTFCNDTVVI PCFVTNMEAQ NTTEVYVKWK FKGRDIYTFD GALNKSTVPT DFSSAKIEVS QLLKGDASLK MDKSDAVSHT GNYTCEVTEL TREGETIIEL KYRVVSWFSP NENILIVIFP I (1-150 aa encoded by BC010016)

靶标信息

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Combinatorial Analysis of AT-Rich Interaction Domain 1A and CD47 in Gastric Cancer Patients Reveals Markers of Prognosis.

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生物信息学

蛋白别名: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

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基因别名: CD47; IAP; MER6; OA3

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UniProt ID: (Human) Q08722

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Entrez Gene ID: (Human) 961

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功能

protein binding thrombospondin receptor activity cell adhesion molecule

参与通路