Invitrogen

CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine5, eBioscience™

CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow)

图: 1 / 5

CD49b (Integrin alpha 2) Antibody (15-5971-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 eFluor® 450 (Product # 48-5941-82) and 0.06 µg of Anti-Mouse CD49b (Integrin alpha 2) PE-Cyanine5.Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

15-5971-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgM, kappa

分类

Monoclonal

类型

Antibody

克隆号

DX5

偶联物

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

激发/发射光谱

568/666 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573070

产品详细信息

Description: The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported: This DX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 15-5971-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Impact of mouse model tumor implantation site on acquired resistance to anti-PD-1 immune checkpoint therapy.

Frontiers in immunology

Denis M,Mathé D,Micoud M,Choffour PA,Grasselly C,Matera EL,Dumontet C

15-5971-82 was used in flow cytometry to demonstrate several differences between SC and orthotopic models at basal state, which tend to become similar after therapy.

Tue Jan 31 00:00:00 EST 2023

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 15-5971-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Microbiota triggers STING-type I IFN-dependent monocyte reprogramming of the tumor microenvironment.

Cell

Lam KC,Araya RE,Huang A,Chen Q,Di Modica M,Rodrigues RR,Lopès A,Johnson SB,Schwarz B,Bohrnsen E,Cogdill AP,Bosio CM,Wargo JA,Lee MP,Goldszmid RS

15-5971-82 was used in Flow cytometry/Cell sorting to uncover a mechanistic link between the microbiota and the innate TME that can be harnessed to improve cancer therapies.

Thu Oct 14 00:00:00 EDT 2021

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

15-5971 was used in Flow cytometry/Cell sorting to study the underlying mechanism by which the targeting of antigen to MHCII on antigen-presenting cells increases antibody responses.

Thu Oct 05 00:00:00 EDT 2023

Differentiation and Protective Capacity of Virus-Specific CD8⁺ T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

Immunity

Tomov VT,Palko O,Lau CW,Pattekar A,Sun Y,Tacheva R,Bengsch B,Manne S,Cosma GL,Eisenlohr LC,Nice TJ,Virgin HW,Wherry EJ

Tue Oct 17 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 15-5971-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection.

Journal of immunology (Baltimore, Md. : 1950)

Palmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC

15-5971 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection.

Sat Aug 15 00:00:00 EDT 2015

生物信息学

蛋白别名: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

查看更多收起

基因别名: CD49B; DX5; Itga2

查看更多收起

UniProt ID: (Mouse) Q62469

查看更多收起

Entrez Gene ID: (Mouse) 16398

查看更多收起

功能

integrin binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion integrin

参与通路