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Invitrogen
CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Functional Grade, eBioscience™
-Functional-Grade-Flow.jpg?time=20220826 "CD51/CD61 (Integrin alpha v beta 3) Antibody in Flow Cytometry (Flow)")
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CD51/CD61 (Integrin alpha v beta 3) Antibody (16-0519-81) in Flow
Staining of platelets with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram), or 0.5 µg of Anti-Human CD51/CD61 (Integrin alpha V beta 3) Purified followed by Anti-Mouse IgG FITC (Product # 11-4011-85) (filled histogram).
-Functional-Grade-Flow.jpg?time=20220826)
-Functional-Grade-Flow.jpg?time=20220826?time=20220826 "CD51/CD61 (Integrin alpha v beta 3) Antibody (16-0519-81) in Flow")
产品信息
16-0519-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
23C6
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_529538
产品详细信息
Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.
Applications Reported: This 23C6 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. 23C6 has also been reported in blocking of some adhesive processes.
Applications Tested: This 23C6 antibody has been tested by flow cytometric analysis of human melanoma cell line and peripheral blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; GPIIIa; integrin alpha V; integrin alpha-5 beta-3; Integrin alpha-V; integrin alphaVbeta3; integrin beta 3; Integrin beta-3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa; Vitronectin receptor; Vitronectin receptor subunit alpha
基因别名: BDPLT16; BDPLT2; CD51; CD61; GP3A; GPIIIa; GT; ITGAV; ITGB3; MSK8; VNRA; VTNR
UniProt ID: (Human) P06756, (Human) P05106
Entrez Gene ID: (Human) 3685, (Human) 3690
virus receptor activity
opsonin binding
fibronectin binding
protease binding
protein kinase C binding
voltage-gated calcium channel activity
protein binding
coreceptor activity
fibroblast growth factor binding
C-X3-C chemokine binding
insulin-like growth factor I binding
neuregulin binding
metal ion binding
transforming growth factor beta binding
extracellular matrix binding
extracellular matrix protein binding
protein disulfide isomerase activity
platelet-derived growth factor receptor binding
enzyme binding
identical protein binding
vascular endothelial growth factor receptor 2 binding
cell adhesion molecule binding
integrin
angiogenesis
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
positive regulation of cell proliferation
negative regulation of macrophage derived foam cell differentiation
negative regulation of lipid storage
cell growth
cell migration
extracellular matrix organization
positive regulation of cell migration
cell-substrate adhesion
negative regulation of lipid transport
positive regulation of osteoblast proliferation
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
endodermal cell differentiation
apolipoprotein A-I-mediated signaling pathway
apoptotic cell clearance
negative regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of cell adhesion
viral entry into host cell
vascular endothelial growth factor receptor signaling pathway
negative regulation of lipoprotein metabolic process
regulation of phagocytosis
leukocyte migration
negative chemotaxis
entry of symbiont into host cell by promotion of host phagocytosis
ERK1 and ERK2 cascade
calcium ion transmembrane transport
extrinsic apoptotic signaling pathway in absence of ligand
regulation of apoptotic cell clearance
negative regulation of entry of bacterium into host cell
negative regulation of extrinsic apoptotic signaling pathway
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
platelet degranulation
cell-substrate junction assembly
blood coagulation
positive regulation of endothelial cell migration
smooth muscle cell migration
platelet activation
positive regulation of vascular endothelial growth factor receptor signaling pathway
activation of protein kinase activity
tube development
wound healing
regulation of bone resorption
mesodermal cell differentiation
positive regulation of peptidyl-tyrosine phosphorylation
angiogenesis involved in wound healing
platelet aggregation
