Invitrogen

CD56 (NCAM) Monoclonal Antibody (CMSSB), PE-Cyanine5, eBioscience™

CD56 (NCAM) Antibody in Flow Cytometry (Flow)

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CD56 (NCAM) Antibody (15-0567-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (left) or Anti-Human CD56 (NCAM) PE-Cyanine5 (right).Cells in the lymphocyte gate were used for analysis.

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产品信息

15-0567-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

CMSSB

偶联物

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

激发/发射光谱

568/666 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573062

产品详细信息

Description: This CMSSB monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells. Staining with CMSSB does not block binding of MEM188 or CB56.

Applications Reported: This CMSSB antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CMSSB antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

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Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Tue Jan 31 00:00:00 EST 2023

Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients.

Cell death & disease

Di Pietro L,Giacalone F,Ragozzino E,Saccone V,Tiberio F,De Bardi M,Picozza M,Borsellino G,Lattanzi W,Guadagni E,Bortolani S,Tasca G,Ricci E,Parolini O

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Fri Sep 16 00:00:00 EDT 2022

Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.

Cell death & disease

Sun Q,Wang Y,Ji H,Sun X,Xie S,Chen L,Li S,Zeng W,Chen R,Tang Q,Zuo J,Hou L,Hosaka K,Lu Y,Liu Y,Ye Y,Yang Y

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Extracellular Vesicle-Mediated Secretion of HLA-E by Trophoblasts Maintains Pregnancy by Regulating the Metabolism of Decidual NK Cells.

International journal of biological sciences

Jiang L,Fei H,Jin X,Liu X,Yang C,Li C,Chen J,Yang A,Zhu J,Wang H,Fei X,Zhang S

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.

The Journal of international medical research

He J,Xu Y,Yi M,Gu C,Zhu Y,Hu G

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Androgen deprivation upregulates SPINK1 expression and potentiates cellular plasticity in prostate cancer.

Nature communications

Tiwari R,Manzar N,Bhatia V,Yadav A,Nengroo MA,Datta D,Carskadon S,Gupta N,Sigouros M,Khani F,Poutanen M,Zoubeidi A,Beltran H,Palanisamy N,Ateeq B

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Mon Jan 20 00:00:00 EST 2020

Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens.

Cell reports

Black JB,McCutcheon SR,Dube S,Barrera A,Klann TS,Rice GA,Adkar SS,Soderling SH,Reddy TE,Gersbach CA

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Impact of FAK Expression on the Cytotoxic Effects of CIK Therapy in Triple-Negative Breast Cancer.

Cancers

Pan MR,Wu CC,Kan JY,Li QL,Chang SJ,Wu CC,Li CL,Ou-Yang F,Hou MF,Yip HK,Luo CW

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Mon Dec 30 00:00:00 EST 2019

Interaction and Mutual Activation of Different Innate Immune Cells Is Necessary to Kill and Clear Hepatitis C Virus-Infected Cells.

Frontiers in immunology

Klöss V,Grünvogel O,Wabnitz G,Eigenbrod T,Ehrhardt S,Lasitschka F,Lohmann V,Dalpke AH

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

Scientific reports

Atkin-Smith GK,Paone S,Zanker DJ,Duan M,Phan TK,Chen W,Hulett MD,Poon IK

15056742 was used in flow cytometry to establish a method to isolate apoptotic bodies from cultured cells to 99% purity

Fri Jan 06 00:00:00 EST 2017

Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.

Proteomics

Bhanu NV,Sidoli S,Garcia BA

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.

Current protocols in cytometry

Soh KT,Tario JD,Colligan S,Maguire O,Pan D,Minderman H,Wallace PK

Published figure using CD56 (NCAM) monoclonal antibody (Product # 15-0567-42) in Flow Cytometry

Wed Jan 06 00:00:00 EST 2016

Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria.

Science (New York, N.Y.)

Sonnenberg GF,Monticelli LA,Alenghat T,Fung TC,Hutnick NA,Kunisawa J,Shibata N,Grunberg S,Sinha R,Zahm AM,Tardif MR,Sathaliyawala T,Kubota M,Farber DL,Collman RG,Shaked A,Fouser LA,Weiner DB,Tessier PA,Friedman JR,Kiyono H,Bushman FD,Chang KM,Artis D

Fri Jun 08 00:00:00 EDT 2012

Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.

Infection and immunity

Schierloh P,Yokobori N,Alemán M,Landoni V,Geffner L,Musella RM,Castagnino J,Baldini M,Abbate E,de la Barrera SS,Sasiain MC

15-0567 was used in Flow cytometry/Cell sorting to study how the interplay between M. tuberculosis and NK cells/APC triggering IFN-gamma may play a beneficial role in tuberculous pleurisy by helping to maintain a type 1 profile.

Thu Nov 01 00:00:00 EDT 2007

生物信息学

蛋白别名: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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基因别名: CD56; MSK39; NCAM; NCAM1

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UniProt ID: (Human) P13591

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Entrez Gene ID: (Human) 4684

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功能

virus receptor activity Ras guanyl-nucleotide exchange factor activity

参与通路

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway