Invitrogen

CD57 Monoclonal Antibody (TB01 (TBO1)), PE, eBioscience™

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CD57 Antibody (12-0577-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgM Isotype Control PE (Product # 12-4752) (left) or Anti-Human CD57 PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0577-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

TB01 (TBO1)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10804531

产品详细信息

Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.

Applications Reported: This TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is expressed on a subset of natural killer (NK) cells and NK T cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.

iScience

McDavid A,Laniewski N,Grier A,Gill AL,Kessler HA,Huyck H,Carbonell E,Holden-Wiltse J,Bandyopadhyay S,Carnahan J,Dylag AM,Topham DJ,Falsey AR,Caserta MT,Pryhuber GS,Gill SR,Scheible KM

Published figure using CD57 monoclonal antibody (Product # 12-0577-42) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir.

eLife

Neidleman J,Luo X,Frouard J,Xie G,Hsiao F,Ma T,Morcilla V,Lee A,Telwatte S,Thomas R,Tamaki W,Wheeler B,Hoh R,Somsouk M,Vohra P,Milush J,James KS,Archin NM,Hunt PW,Deeks SG,Yukl SA,Palmer S,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 12-0577-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients.

Frontiers in immunology

Lim SA,Kim J,Jeon S,Shin MH,Kwon J,Kim TJ,Im K,Han Y,Kwon W,Kim SW,Yee C,Kim SJ,Jang JY,Lee KM

12-0577-42 was used in Flow Cytometry to investigate NK cell defects within pancreatic ductal adenocarcinoma.

Wed Aug 12 00:00:00 EDT 2020

Analysis of NK cell clones obtained using interleukin-2 and gene-modified K562 cells revealed the ability of "senescent" NK cells to lose CD57 expression and start expressing NKG2A.

PloS one

Streltsova MA,Erokhina SA,Kanevskiy LM,Lee DA,Telford WG,Sapozhnikov AM,Kovalenko EI

12-0577-42 was used in Flow Cytometry to demonstrate that in specific conditions terminally differentiated CD57+ human NK cells are able to acquire the CD57– phenotype, which was previously considered impossible.

Mon May 13 00:00:00 EDT 2019

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 12-0577-42) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2017

Identification of Human Memory-Like NK Cells.

Current protocols in cytometry

Kovalenko EI,Streltsova MA,Kanevskiy LM,Erokhina SA,Telford WG

12-0577-42 was used in Flow cytometry/Cell sorting to describe an approach for identification and analysis of adaptive NK cells in human peripheral blood. CD57-positive cells with high expression of activating-receptor NKG2C, increased expression of KIR receptors, lack of co-expression with inhibitory receptor NKG2A, and decreased expression of activating receptor NCR3 (NKp30) all characterize this cell type.

Thu Jan 05 00:00:00 EST 2017

NKG2C(+)CD57(+) Natural Killer Cell Expansion Parallels Cytomegalovirus-Specific CD8(+) T Cell Evolution towards Senescence.

Journal of immunology research

Heath J,Newhook N,Comeau E,Gallant M,Fudge N,Grant M

12-0577 was used in Flow cytometry/Cell sorting to explore the relationship between NKG2C(+)CD57(+) natural killer cells and the adaptive immune response to human cytomegalovirus.

Thu Mar 16 00:00:00 EDT 2017

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD57 monoclonal antibody (Product # 12-0577-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

生物信息学

蛋白别名: Beta-1,3-glucuronyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GlcUAT-P; Glucuronosyltransferase P; HNK-1; Leu-7; LEU7 antigen; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

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基因别名: B3GAT1; CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1

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UniProt ID: (Human) Q9P2W7

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Entrez Gene ID: (Human) 27087

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功能

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity metal ion binding glycosyltransferase

参与通路

carbohydrate metabolic process protein glycosylation glycosaminoglycan metabolic process chondroitin sulfate metabolic process chondroitin sulfate proteoglycan biosynthetic process