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CD63 Antibody (PA5-92370) in ICC/IF
Immunofluorescence analysis of CD63 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD63 Polyclonal Antibody (Product # PA5-92370) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More
产品信息
PA5-92370
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_0017711)
偶联物
Unconjugated
形式
Liquid
浓度
1.07 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2806456
产品详细信息
Immunogen sequence: AGYVFRDKVM SEFNNNFRQQ MENYPKNNHT ASILDRMQAD FKCCGAANYT DWEKIPSMSK NRVPDSCCIN VTVGCGINFN EKAIHKEGCV EKIGGWLRKN V; Positive Samples: A375, 22Rv1, Mouse kidney, Mouse spleen; Cellular Location: Cell membrane, Endosome, Late endosome membrane, Lysosome membrane, Melanosome, Multi-pass membrane protein, multivesicular body
靶标信息
CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30
基因别名: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30
UniProt ID: (Human) P08962
Entrez Gene ID: (Human) 967
positive regulation of receptor internalization
platelet degranulation
cell-matrix adhesion
negative regulation of epithelial cell migration
protein transport
cell migration
epithelial cell differentiation
cellular protein localization
endosome to melanosome transport
positive regulation of cell adhesion
pigment granule maturation
regulation of vascular endothelial growth factor signaling pathway
regulation of rubidium ion transport
positive regulation of integrin-mediated signaling pathway
