Invitrogen

CD63 Monoclonal Antibody (H5C6), PE-Cyanine7, eBioscience™

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CD63 Antibody (25-0639-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD193 (CCR3) PE (Product # 12-1939-42), Anti-Human IgE FITC (Product # 11-6986-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD63 PE-Cyanine7 (purple histogram). CD193+IgE+ cells (left) were used for analysis.

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产品信息

25-0639-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

H5C6

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573358

产品详细信息

Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes. This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells. Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response.

Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

Published figure using CD63 monoclonal antibody (Product # 25-0639-42) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Neuroprotective Effects of Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Extracellular Vesicles in Ischemic Stroke Models.

Biomedicines

Lu G,Su X,Wang L,Leung CK,Zhou J,Xiong Z,Wang W,Liu H,Chan WY

Published figure using CD63 monoclonal antibody (Product # 25-0639-42) in Flow Cytometry

Sun Sep 17 00:00:00 EDT 2023

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Cell death discovery

Zhao G,Ding L,Yu H,Wang W,Wang H,Hu Y,Qin L,Deng G,Xie B,Li G,Qi L

Published figure using CD63 monoclonal antibody (Product # 25-0639-42) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2022

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Sat May 01 00:00:00 EDT 2021

The Interaction between Reactive Peritoneal Mesothelial Cells and Tumor Cells via Extracellular Vesicles Facilitates Colorectal Cancer Dissemination.

Cancers

Serratì S,Porcelli L,Fragassi F,Garofoli M,Di Fonte R,Fucci L,Iacobazzi RM,Palazzo A,Margheri F,Cristiani G,Albano A,De Luca R,Altomare DF,Simone M,Azzariti A

Thu May 20 00:00:00 EDT 2021

Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy.

International journal of molecular sciences

Jabbari N,Nawaz M,Rezaie J

Published figure using CD63 monoclonal antibody (Product # 25-0639-42) in Flow Cytometry

Thu Jul 25 00:00:00 EDT 2019

MicroRNA-containing extracellular vesicles released from endothelial colony-forming cells modulate angiogenesis during ischaemic retinopathy.

Journal of cellular and molecular medicine

Dellett M,Brown ED,Guduric-Fuchs J,O'Connor A,Stitt AW,Medina RJ,Simpson DA

Published figure using CD63 monoclonal antibody (Product # 25-0639-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Urinary exosomes as a novel biomarker for evaluation of α-lipoic acid's protective effect in early diabetic nephropathy.

Journal of clinical laboratory analysis

Sun H,Yao W,Tang Y,Zhuang W,Wu D,Huang S,Sheng H

Published figure using CD63 monoclonal antibody (Product # 25-0639-42) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

25-0639 was used in Flow cytometry/Cell sorting to investigate microvesicles derived from human umbilical cord mesenchymal stem cells, and their ability to attenuate bladder tumour cell growth.

Tue Oct 22 00:00:00 EDT 2013

生物信息学

蛋白别名: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30

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基因别名: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30

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UniProt ID: (Human) P08962

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Entrez Gene ID: (Human) 967

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功能

protein binding protein complex binding

参与通路