Invitrogen

CD63 Monoclonal Antibody (MEM-259), Alexa Fluor™ 488

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CD63 Antibody (MA5-18149) in Flow

Flow cytometry analysis of IgE-activated peripheral human blood stained with anti-human CD63 (MEM-259) Alexa Fluor® 488 Monoclonal antibody (Product # MA5-18149).

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产品信息

MA5-18149

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫细胞化学 (ICC/IF)

Assay-dependent

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流式细胞分析 (Flow)

4 µL/100 µL of whole blood or 10^6 cells

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

MEM-259

抗原

HPB-ALL T cell line

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偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

纯化类型

Size-exclusion chromatography

保存液

PBS with 0.2% BSA

内含物

15mM sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539523

产品详细信息

This antibody reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

靶标信息

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage.

Scientific reports

Kim HJ,Rames MJ,Tassi Yunga S,Armstrong R,Morita M,Ngo ATP,McCarty OJT,Civitci F,Morgan TK,Ngo TTM

MA5-18149 was used in Flow Cytometry to examine the effect of differential centrifugation and a freeze/thaw cycle on extracellular vesicles profiles in blood.

Tue Feb 08 00:00:00 EST 2022

Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage.

Scientific reports

Kim HJ,Rames MJ,Tassi Yunga S,Armstrong R,Morita M,Ngo ATP,McCarty OJT,Civitci F,Morgan TK,Ngo TTM

MA5-18149 was used in Flow Cytometry to examine the effect of differential centrifugation and a freeze/thaw cycle on extracellular vesicles profiles in blood.

Tue Feb 08 00:00:00 EST 2022

Nondestructive production of exosomes loaded with ultrathin palladium nanosheets for targeted bio-orthogonal catalysis.

Nature protocols

Sebastian V,Sancho-Albero M,Arruebo M,Pérez-López AM,Rubio-Ruiz B,Martin-Duque P,Unciti-Broceta A,Santamaría J

MA5-18149 was used in Western Blot to produce high-load catalytically active Pd nanosheet containing exosomes without disrupting their membranes.

Fri Jan 01 00:00:00 EST 2021

Importance of between and within Subject Variability in Extracellular Vesicle Abundance and Cargo when Performing Biomarker Analyses.

Cells

Newman LA,Fahmy A,Sorich MJ,Best OG,Rowland A,Useckaite Z

MA5-18149 was used in Flow cytometry/Cell sorting to evaluate the variability in abundance and cargo of global and liver-specific circulating sEV, within (diurnal) and between individuals in a cohort of healthy subjects.

Wed Feb 24 00:00:00 EST 2021

Microenvironmental pH and Exosome Levels Interplay in Human Cancer Cell Lines of Different Histotypes.

Cancers

Logozzi M,Mizzoni D,Angelini DF,Di Raimo R,Falchi M,Battistini L,Fais S

MA5-18149 was used in Immunocytochemistry to show an increase in exosome release was observed at pH 6.5, indicating tumor acidity and exosome levels represent common cancer phenotypes.

Fri Oct 05 00:00:00 EDT 2018

生物信息学

蛋白别名: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30

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基因别名: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30

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UniProt ID: (Human) P08962

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Entrez Gene ID: (Human) 967

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功能

protein binding protein complex binding

参与通路