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Invitrogen
CD66a (CEACAM1) Monoclonal Antibody (CC1), FITC, eBioscience™
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CD66a (CEACAM1) Antibody (11-0661-82) in Flow
C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82) and 0.06 µg of Mouse IgG1 kappa Isotype Control, FITC (Product # 11-4714-82) (left) or 0.06 µg of CD66a (CEACAM1) Monoclonal Antibody, FITC (right). Cells in the lymphocyte gate were used for analysis.
产品信息
11-0661-82
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
CC1
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2848254
产品详细信息
Description: The monoclonal antibody CC1 recognizes CD66a, also known as carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), biliary glycoprotein, and BPG. Expression of CD66a is found on brush borders, epithelial, and endothelial cells. In hematopoietic cells expression is found abundantly on B cells, as well as some NKs, monocytes, DCs, and granulocytes. Although low levels of mRNA have been identified in T cells in humans, resting mouse T lymphocytes are not reported to express CD66a, as confirmed by lack of staining with CC1 antibody. In humans, expression levels of CD66a have been used to identify malignancies. CD66a plays a key role as a regulator of BCR activation of B lymphocytes.
An alternate allele, CEACAM1b, is expressed in SJL mice; therefore, CC1 does not stain SJL tissue. The monoclonal CC1 has been shown to block viral infection and also enhance B cell proliferation when combined with IgM crosslinking.
Applications Reported: This CC1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
靶标信息
CEACAM1 is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BGP-1; Biliary glycoprotein 1; Biliary glycoprotein D; carcinoembryonic antigen 1; carcinoembryonic antigen 7; Carcinoembryonic antigen-related cell adhesion molecule 1; CD66a; CEA-related cell adhesion molecule 1; hepatitis virus (MHV-4) susceptibility; hepatitis virus receptor; MHV-R; MHVR1; Murine hepatitis virus receptor
基因别名: bb-1; Bgp; Bgp1; C-CAM; Cc1; CD66a; Cea-1; Cea-7; Cea1; Cea7; Ceacam1; Hv-2; Hv2; mCEA1; Mhv-1; MHVR; MHVR1; mmCGM1; mmCGM1a; mmCGM2
UniProt ID: (Mouse) P31809
Entrez Gene ID: (Mouse) 26365
negative regulation of cytokine production
homophilic cell adhesion via plasma membrane adhesion molecules
viral process
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
negative regulation of interleukin-2 production
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of MAP kinase activity
negative regulation by host of viral process
positive regulation by host of viral process
negative regulation by host of viral genome replication
negative regulation of JNK cascade
positive regulation of JNK cascade
viral entry into host cell
Peyer's patch development
positive regulation of immunoglobulin secretion
positive regulation of NFAT protein import into nucleus
positive regulation of activation-induced cell death of T cells
positive regulation of CD4-positive, alpha-beta T cell activation
positive regulation of CD4-positive, alpha-beta T cell proliferation
positive regulation of CD8-positive, alpha-beta T cell activation
