Invitrogen

CD68 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他89个CD68抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (7)
高级验证 (1)

CD68 Antibody in Immunocytochemistry (ICC/IF)

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CD68 Antibody (PA5-109344) in ICC/IF

Immunofluorescence analysis of Cd68 was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD68 Polyclonal Antibody (Product # PA5-109344) (1:100 dilution) at room temperature in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus ... View More

CD68 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD68 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD68 Polyclonal Antibody (Product # PA5-109344), shows expression of CD68 in K562 cells in comparison to Jurkat cells. Relative Expression. {RE}

产品信息

PA5-109344

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within Human CD68 aa 25-74

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2854755

产品详细信息

Positive Control: Human lung tissue lysates, SiHa, human spleen tissue, human colon tissue, human lung carcinoma tissue, A549.

靶标信息

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。