Invitrogen

CD68 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD68 Antibody in Immunocytochemistry (ICC/IF)

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CD68 Antibody (PA5-83940) in ICC/IF

Nature communications 2022 - Fig. 6 Changes in alveolar macrophage population composition in COPD. A UMAPs of control and COPD alveolar macrophage cells color labelled by Louvain cluster (left) and disease status (right). B Percent makeup of alveolar macrophage across all nine Louvain clusters per subject, as assessed by single-cell RNA sequencing (scRNAseq) from 14 lungs with advanced COPD and 13 control donor lungs. Boxes represent m... View More

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CD68 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 mg ATCC cells were transfected with, control siRNA, CD68 siRNA probe #1, and CD68 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-CD68 Polyclonal Antibody (Product # PA5-83940) used at a dilution of 0.4 µg/mL. Anti-CD68 was used as a loading control. {KD}

产品信息

PA5-83940

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化 (IHC)

1:2,500-1:5,000

免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human CD68. Recombinant protein control fragment (Product #RP-100954).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.10 mg/mL

纯化类型

Anitgen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2791092

产品详细信息

Immunogen sequence: SPTSKETIGD YTWTNGSQPC VHLQAQIQIR VMYTTQGGGE AWGISVLNPN KTKVQGSCEG AHPHLLLSFP YG

靶标信息

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Characterization of the COPD alveolar niche using single-cell RNA sequencing.

Nature communications

Sauler M,McDonough JE,Adams TS,Kothapalli N,Barnthaler T,Werder RB,Schupp JC,Nouws J,Robertson MJ,Coarfa C,Yang T,Chioccioli M,Omote N,Cosme C,Poli S,Ayaub EA,Chu SG,Jensen KH,Gomez JL,Britto CJ,Raredon MSB,Niklason LE,Wilson AA,Timshel PN,Kaminski N,Rosas IO

PA5-83940 was used in Immunocytochemistry, Immunohistochemistry (Paraffin) to highlight cell-specific mechanisms involved in the pathobiology of advanced COPD.

Tue Jan 25 00:00:00 EST 2022

Characterization of the COPD alveolar niche using single-cell RNA sequencing.

Nature communications

PA5-83940 was used in Immunocytochemistry, Immunohistochemistry (Paraffin) to highlight cell-specific mechanisms involved in the pathobiology of advanced COPD.