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Invitrogen
CD68 Monoclonal Antibody (514H12)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
图: 1 / 4
CD68 Antibody (MA1-80133) in ICC/IF
Immunofluorescence analysis of CD68 was performed using 70% confluent log phase THP-1 cells differentiated into Macrophage (M0 phase). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD68 Mouse Monoclonal Antibody (514H12) (Product # MA1-80133) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibo... View More
产品信息
MA1-80133
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
514H12
抗原
Fusion protein of external domain of human CD68.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
tissue culture supernatant
内含物
<0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_929283
产品详细信息
Heat-mediated antigen retrieval using Tris/EDTA (pH 8) is recommended for the staining of paraffin sections. The epitope recognized by clone 514H12 is sensitive to peroxide and so any peroxidase-blocking steps should be performed after incubation with this antibody. A suggested positive control for immunohistochemical applications is human tonsil.
Mouse anti Human CD68 antibody, clone 514H12 recognizes the human CD68 cell surface antigen, a approximately 110 kDa glycoprotein primarily expressed by macrophages and monocytes.
靶标信息
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD68; CD68 antigen; Gp110; macrophage antigen CD68; Macrosialin; scavenger receptor class D, member 1
基因别名: CD68; GP110; LAMP4; SCARD1
UniProt ID: (Human) P34810
Entrez Gene ID: (Human) 968
