Invitrogen

CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), PE-Cyanine7, eBioscience™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

16个已发表图片

15 篇参考文献

查看其他89个CD68抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 18

CD68 Antibody (25-0689-42) in Flow

Intracellular staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4732-81) (blue histogram) or Anti-Human CD68 PE-Cyanine7 (purple histogram). Cells in the monocyte gate were used for analysis.

Product Image Gallery

CD68 Antibody — Antibody clone (eBioY1/82A (Y1/82A)) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in eBioY1/82A (Y1/82A) KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD68 antibody (eBioY1/82A (Y1/82A)). Yellow histogram represents staining with the isotype control. {KO}

产品信息

25-0689-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

查看14篇已发表文章 14篇已发表文章

免疫电镜技术 (IM)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioY1/82A (Y1/82A)

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573364

产品详细信息

Description: The eBioY1/82 antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on it's homolgy and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.

Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.

Applications Reported: This eBioY1/82A (Y1/82A) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioY1/82A (Y1/82A) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Activation of the JNK/COX-2/HIF-1α axis promotes M1 macrophage via glycolytic shift in HIV-1 infection.

Life science alliance

Zhang J,Yuan Z,Li X,Wang F,Wei X,Kang Y,Mo C,Jiang J,Liang H,Ye L

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2023

NLRP3 in tumor-associated macrophages predicts a poor prognosis and promotes tumor growth in head and neck squamous cell carcinoma.

Cancer immunology, immunotherapy : CII

Chen L,Wan SC,Mao L,Huang CF,Bu LL,Sun ZJ

25-0689-42 was used in Flow cytometry/Cell sorting to find that NLRP3 was highly expressed in tumor-associated macrophages (TAMs) in both mouse and human HNSCC, and the expression of NLRP3 was positively correlated with the density of TAMs according to immunohistochemistry, immunofluorescence, and flow cytometry analyses.

Thu Jun 01 00:00:00 EDT 2023

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

CNS neuroscience & therapeutics

Zhang H,Wang Y,Zhao Y,Liu T,Wang Z,Zhang N,Dai Z,Wu W,Cao H,Feng S,Zhang L,Cheng Q,Liu Z

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

The role of CD68+ macrophage in classical Hodgkin lymphoma patients from Egypt.

Diagnostic pathology

Mohamed O,El Bastawisy A,Allahlobi N,Abdellateif MS,Zekri ARN,Shaarawy S,Korany Z,Mohanad M,Bahnassy AA

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Tue Feb 04 00:00:00 EST 2020

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages.

Materials (Basel, Switzerland)

Schmitz T,Jannasch M,Weigel T,Moseke C,Gbureck U,Groll J,Walles H,Hansmann J

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Immunomicroscopy

Wed Mar 04 00:00:00 EST 2020

Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.

Science advances

Izadi D,Layton TB,Williams L,McCann F,Cabrita M,Espirito Santo AI,Xie W,Fritzsche M,Colin-York H,Feldmann M,Midwood KS,Nanchahal J

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Intrahepatic macrophage populations in the pathophysiology of primary sclerosing cholangitis.

JHEP reports : innovation in hepatology

Chen YY,Arndtz K,Webb G,Corrigan M,Akiror S,Liaskou E,Woodward P,Adams DH,Weston CJ,Hirschfield GM

25-0689 was used in Flow cytometry/Cell sorting to show CD68+CD206+ macrophages occur in greater frequency with higher expression of TGR-5 in primary sclerosing cholangitis.

Fri Nov 01 00:00:00 EDT 2019

Multi-cellular human bronchial models exposed to diesel exhaust particles: assessment of inflammation, oxidative stress and macrophage polarization.

Particle and fibre toxicology

Ji J,Upadhyay S,Xiong X,Malmlöf M,Sandström T,Gerde P,Palmberg L

25-0689 was used in Flow cytometry/Cell sorting to identify interaction of primary bronchial epithelial cells with macrophages in response to diesel exhaust particles aid macrophage phenotypic alteration towards M2-subtypes.

Wed May 02 00:00:00 EDT 2018

Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.

Journal of neurovirology

Garcia-Mesa Y,Jay TR,Checkley MA,Luttge B,Dobrowolski C,Valadkhan S,Landreth GE,Karn J,Alvarez-Carbonell D

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.

Bioscience reports

Zhong Y,Yi C

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD68 monoclonal antibody (Product # 25-0689-42) in Flow Cytometry

Fri Dec 02 00:00:00 EST 2016

Local iron homeostasis in the breast ductal carcinoma microenvironment.

BMC cancer

Marques O,Porto G,Rêma A,Faria F,Cruz Paula A,Gomez-Lazaro M,Silva P,Martins da Silva B,Lopes C

25-0689 was used in Flow cytometry/Cell sorting to analyse the iron-profiles of epithelial cells, lymphocytes and macrophages in normal human breast and ductal carcinoma samples.