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Invitrogen
CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), PE-eFluor™ 610, eBioscience™
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
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CD68 Antibody (61-0689-42) in Flow
Intracellular staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PE-eFluor® 610 (Product # 61-4732-82) (blue histogram) or Anti-Human CD68 PE-eFluor® 610 (purple histogram) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the monocyte gate were used for analysis.
产品信息
61-0689-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBioY1/82A (Y1/82A)
偶联物
激发/发射光谱
565/606 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574570
产品详细信息
Description: The eBioY1/82 antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on it's homolgy and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.
Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.
Applications Reported: This eBioY1/82A (Y1/82A) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBioY1/82A (Y1/82A) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
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生物信息学
蛋白别名: CD68; CD68 antigen; Gp110; macrophage antigen CD68; Macrosialin; scavenger receptor class D, member 1
基因别名: CD68; GP110; LAMP4; SCARD1
UniProt ID: (Human) P34810
Entrez Gene ID: (Human) 968
