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Invitrogen
CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), eBioscience™
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CD7 Antibody (14-0079-82) in Flow
Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-human CD7 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82). Cells in the lymphocyte gate were used for analysis.
产品信息
14-0079-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBio124-1D1 (124-1D1)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_823132
产品详细信息
Description: The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 and Leu9. CD7, a 40 kD receptor, is a member of the immunoglobulin gene superfamily. The N-terminal amino acid sequence (aa1-107) is highly homologous to Ig kappa light chain sequence; while the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T lymphocytes, and T cell leukemias. It is also found on natural killer cells, a small suppopulation of normal B cells and on maligant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity, as measured by calcium flux, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.
A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells that express CD45RA and HLA-DR and high levels of the CD7 has been reported. These cells possess the capacity for lymphopoiesis. They can generate B-cells, natural killer cells, and dendritic cells but do not possess the capacity to develop into myeloid cells or erythroid cells. The CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells.
Furthermore, it has been suggested that CD7 co-operates with CD28 during Treg function, as mice deficient in both CD28 and CD7 have reduced total numbers of Tregs and these Tregs have reduced suppressive activity.
Applications Reported: This eBio124-1D1 (124-1D1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio124-1D1 (124-1D1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Expression of CD7 is an important marker used in leukemia diagnostics.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD7; CD7 antigen (p41); GP40; p41 protein; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41
基因别名: CD7; GP40; LEU-9; Tp40; TP41
UniProt ID: (Human) P09564
Entrez Gene ID: (Human) 924
