Invitrogen

CD71 (Transferrin Receptor) Recombinant Rabbit Monoclonal Antibody (JF0956)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他109个Transferrin Receptor (CD71)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (7)
高级验证 (1)

CD71 (Transferrin Receptor) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD71 (Transferrin Receptor) Antibody (MA5-32500) in IHC (P)

Immunohistochemistry analysis of CD71 (Transferrin Receptor) with paraffin-embedded human lung carcinoma. The tissues were blocked in 5% BSA (30 min, room temp), washed with ddH2O and PBS, incubated with recombinant monoclonal CD71 (Transferrin Receptor) (Product # MA5-32500) at a dilution of 1:50 (30 min, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained wit... View More

CD71 (Transferrin Receptor) Antibody in Western Blot (WB)

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

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产品信息

MA5-32500

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

免疫组化（石蜡） (IHC (P))

1:50

流式细胞分析 (Flow)

1:50-1:100

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产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JF0956

抗原

Synthetic peptide within Human CD71 aa 22-60

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809777

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。