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CD74 Antibody (11-0748-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD74 FITC (right). Cells in the lymphocyte gate were used for analysis.
产品信息
11-0748-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
5-329
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2043844
产品详细信息
Description: This 5-329 monoclonal antibody recognizes human CD74, also known as the MHC Class II-associated invariant chain. A 33-43 kDa nonpolymorphic type II membrane protein, CD74 is highly expressed on B cells and subsets of activated T cells, Langerhans cells, dendritic cells, and epithelial cells. Monocytes and macrophages also express CD74, but at lower levels. CD74 acts as a chaperone of MHC Class II (HLA-DR) proteins, promoting their translocation from the ER to endocytic compartments during antigen presentation. Activation of NFkappaB and ERK has been shown following CD74 interaction with CD44 and binding to the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 also plays a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells. CD74 has also been demonstrated to interact with CXCR2 and CXCR4.
Applications Reported: This 5-329 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5-329 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; MHC HLA-DR gamma chain; p33
基因别名: CD74; DHLAG; HLADG; Ia-GAMMA; II
UniProt ID: (Human) P04233
Entrez Gene ID: (Human) 972
beta-amyloid binding
cytokine receptor activity
protein binding
cytokine binding
MHC class II protein complex binding
macrophage migration inhibitory factor binding
MHC class II protein binding
MHC class II protein binding, via antigen binding groove
identical protein binding
protein binding involved in protein folding
nitric-oxide synthase binding
scaffold/adaptor protein
activation of MAPK activity
prostaglandin biosynthetic process
positive regulation of cytokine-mediated signaling pathway
positive regulation of dendritic cell antigen processing and presentation
negative regulation of peptide secretion
positive regulation of type 2 immune response
negative regulation of mature B cell apoptotic process
protein complex assembly
intracellular protein transport
defense response
signal transduction
cell proliferation
immunoglobulin mediated immune response
antigen processing and presentation of endogenous antigen
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of B cell proliferation
macrophage migration inhibitory factor signaling pathway
regulation of macrophage activation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
T cell selection
positive thymic T cell selection
negative thymic T cell selection
negative regulation of T cell differentiation
positive regulation of T cell differentiation
positive regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
chaperone mediated protein folding requiring cofactor
positive regulation of macrophage cytokine production
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
