Invitrogen

CD8 Monoclonal Antibody (3B5), TRI-COLOR™

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CD8 Antibody (MHCD0806) in Flow

Human peripheral blood lymphocytes were stained using TRI-COLOR® of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.

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产品信息

MHCD0806

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

3B5

抗原

Human CD8

偶联物

TRI-COLOR™ TRI-COLOR™ TRI-COLOR™

激发/发射光谱

567/671 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10372207

产品详细信息

The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5® conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Analyte Specific Reagent

How to use the Panel Builder

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Product References

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

MHCD0806 was used in Flow cytometry/Cell sorting to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

Intermediate DNA methylation is a conserved signature of genome regulation.

Nature communications

Elliott G,Hong C,Xing X,Zhou X,Li D,Coarfa C,Bell RJ,Maire CL,Ligon KL,Sigaroudinia M,Gascard P,Tlsty TD,Harris RA,Schalkwyk LC,Bilenky M,Mill J,Farnham PJ,Kellis M,Marra MA,Milosavljevic A,Hirst M,Stormo GD,Wang T,Costello JF

MHCD0806 was used in flow cytometry to show that intermediate DNA methylation is a conserved signature of gene regulation and exon usage

Wed Feb 18 00:00:00 EST 2015

Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody.

The Journal of infectious diseases

Luiza-Silva M,Campi-Azevedo AC,Batista MA,Martins MA,Avelar RS,da Silveira Lemos D,Bastos Camacho LA,de Menezes Martins R,de Lourdes de Sousa Maia M,Guedes Farias RH,da Silva Freire M,Galler R,Homma A,Leite Ribeiro JG,Campos Lemos JA,Auxiliadora-Martins M,Eloi-Santos SM,Teixeira-Carvalho A,Martins-Filho OA

MHCD0806 was used in flow cytometry to discuss the live attenuated yellow fever vaccines.

Thu Sep 15 00:00:00 EDT 2011

The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.

Journal of cellular and molecular medicine

Elsner L,Flügge PF,Lozano J,Muppala V,Eiz-Vesper B,Demiroglu SY,Malzahn D,Herrmann T,Brunner E,Bickeböller H,Multhoff G,Walter L,Dressel R

MHCD0806 was used in Flow cytometry/Cell sorting to elucidate how the activity of two stress-inducible immunological danger signals leads to activation and enhanced cytotoxicity of human natural killer cells against tumour cells.

Thu Apr 01 00:00:00 EDT 2010

Superior T cell activation by ESAT-6 as compared with the ESAT-6-CFP-10 complex.

International immunology

Marei A,Ghaemmaghami A,Renshaw P,Wiselka M,Barer M,Carr M,Ziegler-Heitbrock L

MHCD0806 was used in Flow cytometry/Cell sorting to assess T cell activation in response to the ESAT-6-CFP-10 complex, compared with the individual proteins.

Tue Nov 01 00:00:00 EST 2005

Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.

The Journal of biological chemistry

Venkatesan S,Petrovic A,Van Ryk DI,Locati M,Weissman D,Murphy PM

MHCD0806 was used in flow cytometry to determine how CCR5 expression is reduced in cells from ccr5Delta32 heterozygotes.

Fri Jan 18 00:00:00 EST 2002

A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.

The Journal of biological chemistry

Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM

MHCD0806 was used in flow cytometry to characterize CCR5 truncations.

Fri Oct 26 00:00:00 EDT 2001

Analysis of T-cell clones in systemic lupus erythematosus.

Haematologica

Converso M,Bertero MT,Vallario A,Caligaris-Cappio F

MHCD0806 was used in flow cytometry to investigate the role Th cells and their relationship with Th2 cells in human systemic lupus erythematosus.

Tue Feb 01 00:00:00 EST 2000

Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.

Journal of virology

Giavedoni LD,Velasquillo MC,Parodi LM,Hubbard GB,Hodara VL

MHCD0806 was used in flow cytometry to examine the innate and adaptive immune responses of rhesus macaques infected with SIVmac251.

Tue Feb 01 00:00:00 EST 2000

A functional link for major TCR expansions in healthy adults caused by persistent Epstein-Barr virus infection.

The Journal of clinical investigation

Silins SL,Cross SM,Krauer KG,Moss DJ,Schmidt CW,Misko IS

MHCD0806 was used in Flow cytometry/Cell sorting to study the effects of persistent infection with the ubiquitous Epstein-Barr virus on the memory repertoire of healthy virus carriers.

Thu Oct 15 00:00:00 EDT 1998

生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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基因别名: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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功能

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

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