Invitrogen

CD86 Monoclonal Antibody (GL-1), FITC

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CD86 Antibody (MA1-10300) in Flow

Separation of murine CD86 positive myeloid cells (red-filled) from CD86 negative lymphoid cells (black-dashed) in flow cytometry analysis (surface staining) murine splenocyte suspension stained using anti-mouse CD86 (GL-1) FITC Monoclonal antibody (Product # MA1-10300) (concentration in sample 0.33 µg/mL).

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产品信息

MA1-10300

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1-2 µg/mL

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

GL-1

抗原

LPS-activated CBA/Cs mouse splenic B cells

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偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11153707

产品详细信息

This antibody reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

靶标信息

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

miR‑382 inhibits breast cancer progression and metastasis by affecting the M2 polarization of tumor‑associated macrophages by targeting PGC‑1α.

International journal of oncology

Zhou H,Gan M,Jin X,Dai M,Wang Y,Lei Y,Lin Z,Ming J

MA1-10300 was used in Flow cytometry/Cell sorting to suggest that miR‚382 may alter the metabolic status of macrophages by targeting PGC‚1α, thereby decreasing the proportion of TAMs with the M2 phenotype, and inhibiting the progression and metastasis of breast cancer.

Sat Oct 01 00:00:00 EDT 2022

miR‑382 inhibits breast cancer progression and metastasis by affecting the M2 polarization of tumor‑associated macrophages by targeting PGC‑1α.

International journal of oncology

Zhou H,Gan M,Jin X,Dai M,Wang Y,Lei Y,Lin Z,Ming J

Sat Oct 01 00:00:00 EDT 2022

Inhibition of the Fibrinogen-Like Protein 2:FcγRIIB/RIII immunosuppressive pathway enhances antiviral T-cell and B-cell responses leading to clearance of lymphocytic choriomeningitis virus clone 13.

Immunology

Luft O,Khattar R,Farrokhi K,Ferri D,Yavorska N,Zhang J,Sadozai H,Adeyi O,Chruscinski A,Levy GA,Selzner N

MA1-10300 was used in Flow cytometry/Cell sorting to demonstrate an important role for FGL2 in viral immune evasion and provide a rationale to target FGL2 to treat patients with chronic viral infection.

Sun Jul 01 00:00:00 EDT 2018

TFAM is a novel mediator of immunogenic cancer cell death.

Oncoimmunology

Yang M,Li C,Zhu S,Cao L,Kroemer G,Zeh H,Tang D,Kang R

MA1-10300 was used in Flow cytometry/Cell sorting to show that spautin-1 may stimulate an apoptotic pathway that results in immunogenic cell death, in TFAM- and AGER-dependent fashion.

Tue Jan 12 00:00:00 EST 2021

生物信息学

蛋白别名: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

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基因别名: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

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UniProt ID: (Mouse) P42082

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Entrez Gene ID: (Mouse) 12524

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功能

receptor binding immunoglobulin receptor superfamily

参与通路

toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide