Invitrogen

CD9 Recombinant Rabbit Monoclonal Antibody (SA35-08)

CD9 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

CD9 Antibody (MA5-31980) in ICC/IF

Immunocytochemical analysis of CD9 in SW480 cells using a CD9 Monoclonal antibody (Product # MA5-31980) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

CD9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

MA5-31980

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:50

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SA35-08

抗原

Synthetic peptide within Human CD9 aa 179-228

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809274

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

MA5-31980 was used in Western Blotting to find that ADSCs-EVs transferred miR-22-3p to pneumonocytes and reduced apoptosis, inflammation, and oxidative stress through KDM6B/HMGA2, mitigating OSAHS-LI progression.

Wed Mar 01 00:00:00 EST 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

Wed Mar 01 00:00:00 EST 2023

Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/β-Catenin Pathway.

Analytical cellular pathology (Amsterdam)

Yu Z,Liu J,Fan Q,Yu J,Ren X,Wang X

Published figure using CD9 recombinant monoclonal antibody (Product # MA5-31980) in Western Blot

Mon Apr 04 00:00:00 EDT 2022

Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma.

Molecular medicine reports

Miao N,Cai W,Ding S,Liu Y,Chen W,Sun T

MA5-31980 was used in Western Blotting to identify potential exosomal miRNAs that respond to radiotherapy, plasma exosomal miRNAs from ESCC patients undergoing radiotherapy were isolated and sequenced.

Thu Sep 01 00:00:00 EDT 2022

Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma.

Molecular medicine reports

Miao N,Cai W,Ding S,Liu Y,Chen W,Sun T

Thu Sep 01 00:00:00 EDT 2022

Cell death & disease

Zhang M,Gong W,Zhang D,Ji M,Chen B,Chen B,Li X,Zhou Y,Dong C,Wen G,Zhan X,Wu X,Cui L,Feng Y,Wang S,Yuan H,Xu E,Xia M,Verkhratsky A,Li B

Published figure using CD9 recombinant monoclonal antibody (Product # MA5-31980) in Western Blot

Mon Apr 25 00:00:00 EDT 2022

A light-inducible protein clustering system for in vivo analysis of α-synuclein aggregation in Parkinson disease.

PLoS biology

Bérard M,Sheta R,Malvaut S,Rodriguez-Aller R,Teixeira M,Idi W,Turmel R,Alpaugh M,Dubois M,Dahmene M,Salesse C,Lamontagne-Proulx J,St-Pierre MK,Tavassoly O,Luo W,Del Cid-Pellitero E,Qazi R,Jeong JW,Durcan TM,Vallières L,Tremblay ME,Soulet D,Lévesque M,Cicchetti F,Fon EA,Saghatelyan A,Oueslati A

MA5-31980 was used in Immunocytochemistry-immunofluorescence to provide a new tool for the generation, visualization, and dissection of the role of α-syn aggregation in PD.

Tue Mar 01 00:00:00 EST 2022

Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury.

International journal of molecular sciences

Qian J,Park DJ,Perrott S,Patel P,Eliceiri BP

Published figure using CD9 recombinant monoclonal antibody (Product # MA5-31980) in Western Blot

Mon Mar 29 00:00:00 EDT 2021

Macrophage secretion of miR-106b-5p causes renin-dependent hypertension.

Nature communications

Oh J,Matkovich SJ,Riek AE,Bindom SM,Shao JS,Head RD,Barve RA,Sands MS,Carmeliet G,Osei-Owusu P,Knutsen RH,Zhang H,Blumer KJ,Nichols CG,Mecham RP,Baldán Á,Benitez BA,Sequeira-Lopez ML,Gomez RA,Bernal-Mizrachi C

MA5-31980 was used in Western Blotting to confirm that macrophage miR-106b-5p secretion from impaired vitamin D receptor signalling causes inflammation-induced hypertension.

Wed Sep 23 00:00:00 EDT 2020

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Nature communications

Dinh PC,Paudel D,Brochu H,Popowski KD,Gracieux MC,Cores J,Huang K,Hensley MT,Harrell E,Vandergriff AC,George AK,Barrio RT,Hu S,Allen TA,Blackburn K,Caranasos TG,Peng X,Schnabel LV,Adler KB,Lobo LJ,Goshe MB,Cheng K

MA5-31980 was used in Western Blotting to show that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis.

Fri Feb 28 00:00:00 EST 2020

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Nature communications

Fri Feb 28 00:00:00 EST 2020

生物信息学

蛋白别名: 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin-29; Tetraspanin29; Tspan-29

查看更多收起

基因别名: BTCC-1; CD9; DRAP-27; GIG2; MIC3; MRP-1; TSPAN-29; TSPAN29

查看更多收起

UniProt ID: (Human) P21926, (Mouse) P40240, (Rat) P40241

查看更多收起

Entrez Gene ID: (Human) 928, (Mouse) 12527, (Rat) 24936

查看更多收起

功能

integrin binding protein binding

参与通路