Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (DX2), PE-Cyanine7, eBioscience™

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)

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CD95 (APO-1/Fas) Antibody (25-0959-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD95 (Fas/APO-1) PE-Cyanine7 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

25-0959-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

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产品规格

种属反应

Dog, Human

已发表种属

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

DX2

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1548716

产品详细信息

Description: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.

The DX2 monoclonal is reported to recognize dog/canine CD95.

Applications Reported: This DX2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Genetic disruption of oncogenic Kras sensitizes lung cancer cells to Fas receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Mou H,Moore J,Malonia SK,Li Y,Ozata DM,Hough S,Song CQ,Smith JL,Fischer A,Weng Z,Green MR,Xue W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Tue Apr 04 00:00:00 EDT 2017

Genetic disruption of oncogenic Kras sensitizes lung cancer cells to Fas receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Mou H,Moore J,Malonia SK,Li Y,Ozata DM,Hough S,Song CQ,Smith JL,Fischer A,Weng Z,Green MR,Xue W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Tue Apr 04 00:00:00 EDT 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Transmembrane TNF-α promotes activation-induced cell death by forward and reverse signaling.

Oncotarget

Zhang M,Wang J,Jia L,Huang J,He C,Hu F,Yuan L,Wang G,Yu M,Li Z

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Fri Sep 08 00:00:00 EDT 2017

Association of lymph-node antigens with lower Gag-specific central-memory and higher Env-specific effector-memory CD8(+) T-cell frequencies in a macaque AIDS model.

Scientific reports

Ishii H,Matsuoka S,Nomura T,Nakamura M,Shiino T,Sato Y,Iwata-Yoshikawa N,Hasegawa H,Mizuta K,Sakawaki H,Miura T,Koyanagi Y,Naruse TK,Kimura A,Matano T

25-0959 was used in Flow cytometry/Cell sorting to imply that core and envelope antigen-specific CD8(+) T cells show different patterns of interactions with HIV/SIV-infected cell.

Mon Jul 25 00:00:00 EDT 2016

Target cell availability, rather than breast milk factors, dictates mother-to-infant transmission of SIV in sooty mangabeys and rhesus macaques.

PLoS pathogens

Chahroudi A,Cartwright E,Lee ST,Mavigner M,Carnathan DG,Lawson B,Carnathan PM,Hashempoor T,Murphy MK,Meeker T,Ehnert S,Souder C,Else JG,Cohen J,Collman RG,Vanderford TH,Permar SR,Derdeyn CA,Villinger F,Silvestri G

25-0959 was used in Flow cytometry/Cell sorting to elucidate the factors that prevent the vertical transmission of SIV in sooty mangabeys.

Sat Mar 01 00:00:00 EST 2014

Target cell availability, rather than breast milk factors, dictates mother-to-infant transmission of SIV in sooty mangabeys and rhesus macaques.

PLoS pathogens

25-0959 was used in Flow cytometry/Cell sorting to elucidate the factors that prevent the vertical transmission of SIV in sooty mangabeys.

Sat Mar 01 00:00:00 EST 2014

Effects of gastrokine‑2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways.

Molecular medicine reports

Shi LS,Wang H,Wang F,Feng M,Wang M,Guan WX

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Dompe N,Rivers CS,Li L,Cordes S,Schwickart M,Punnoose EA,Amler L,Seshagiri S,Tang J,Modrusan Z,Davis DP

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Tue Oct 25 00:00:00 EDT 2011

Cross-reactive T cells are involved in rapid clearance of 2009 pandemic H1N1 influenza virus in nonhuman primates.

PLoS pathogens

Weinfurter JT,Brunner K,Capuano SV,Li C,Broman KW,Kawaoka Y,Friedrich TC

25-0959 was used in Flow cytometry/Cell sorting to suggest that cross-reactive T cell responses can mediate early clearance of an antigenically novel influenza virus in primates.

Tue Nov 01 00:00:00 EDT 2011

Strong viremia control in vaccinated macaques does not prevent gradual Th17 cell loss from central memory.

Vaccine

Demberg T,Ettinger AC,Aladi S,McKinnon K,Kuddo T,Venzon D,Patterson LJ,Phillips TM,Robert-Guroff M

25-0959 was used in Flow cytometry/Cell sorting to investigate a vaccine regimen that potently reduced viremia post-challenge preserved Th17 and Tc17 cells, thus benefiting in the absence of sterilizing protection.

Thu Aug 11 00:00:00 EDT 2011

Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.

Retrovirology

Li B,Berry N,Ham C,Ferguson D,Smith D,Hall J,Page M,Quartey-Papafio R,Elsley W,Robinson M,Almond N,Stebbings R

25-0959 was used in Flow cytometry/Cell sorting to investigate whether target cell depletion contributes to the protection of infection with wild-type simian immunodeficiency virus.

Thu Feb 03 00:00:00 EST 2011

CD40·FasL and CTLA-4·FasL fusion proteins induce apoptosis in malignant cell lines by dual signaling.

The American journal of pathology

Orbach A,Rachmilewitz J,Shani N,Isenberg Y,Parnas M,Huang JH,Tykocinski ML,Dranitzki-Elhalel M

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 25-0959-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2010

生物信息学

蛋白别名: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

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基因别名: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

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UniProt ID: (Human) P25445

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Entrez Gene ID: (Dog) 486469, (Human) 355

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功能

protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

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