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CD97 Antibody (PA5-80320) in ICC/IF
Immunofluorescence staining of CD97 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD97 Polyclonal Antibody (Product # PA5-80320, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
产品信息
PA5-80320
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human CD97 Protein (Met1-Gln398)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2787649
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human CD97.
靶标信息
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Adhesion G protein-coupled receptor E5; CD97; CD97 molecule; Leukocyte antigen CD97; seven transmembrane helix receptor; seven-span transmembrane protein; seven-transmembrane, heterodimeric receptor associated with inflammation
基因别名: ADGRE5; CD97; TM7LN1
UniProt ID: (Human) P48960
Entrez Gene ID: (Human) 976
