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Invitrogen
CD99 Monoclonal Antibody (HO36-1.1)
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图: 1 / 6
CD99 Antibody (MA5-12954) in ICC/IF
Immunofluorescent analysis of CD99 (green) showing staining in the membrane of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD99 monoclonal antibody (Product # MA5-12954) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugate... View More
产品信息
MA5-12954
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
HO36-1.1
抗原
Purified E-rosette forming cells from human peripheral blood lymphocytes
偶联物
形式
Liquid
浓度
0.4 mg/mL
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA5-12954 targets CD99 in WB, IF, FACS and IHC (P) applications and shows reactivity with Human and Rat samples. This antibody is not suitable for Mouse spleen cells in Western blot analysis.
The MA5-12954 immunogen is purified E-rosette forming cells from human peripheral blood lymphocytes.
靶标信息
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
