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Abnova

CDK4 Monoclonal Antibody (1E1)

查看其他52个CDK4抗体

说明书

实验方案

技术支持

货号 H00001019-M10

CNY 5,298.00

100 µg

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说明书

实验方案

技术支持

抗体检测数据 (2)

Application

图: 1 / 2

CDK4 Antibody (H00001019-M10) in WB

Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M10), clone 1E1.

Lane 1: CDK4 transfected lysate(33.7 KDa).
Lane 2: Non-transfected lysate.

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产品信息

H00001019-M10

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

酶联免疫吸附实验 (ELISA)

3 ng/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

1E1

抗原

CDK4 (AAH03644, 1 a.a. approximately 303 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated

形式

Liquid

浓度

See Label

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Sequence of this protein is as follows: MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP ISTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDREIKVT LVFEHVDQDL RTYLDKAPPP GLPAETIKDL MRQFLRGLDF LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE

靶标信息

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cell division protein kinase 4; Cyclin-dependent kinase 4; MGC14458; PSK-J3

基因别名: CDK4; CMM3; PSK-J3

UniProt ID: (Human) P11802

Entrez Gene ID: (Human) 1019

功能

cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding cyclin-dependent protein serine/threonine kinase regulator activity cyclin binding protein complex binding non-receptor serine/threonine protein kinase

参与通路

G1/S transition of mitotic cell cycle lens development in camera-type eye protein phosphorylation signal transduction circadian rhythm positive regulation of cell proliferation response to toxic substance response to lead ion regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle organ regeneration response to testosterone response to drug positive regulation of apoptotic process positive regulation of translation positive regulation of cell cycle positive regulation of cell size regulation of protein kinase activity positive regulation of fibroblast proliferation cell division response to hyperoxia negative regulation of cell cycle arrest