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Invitrogen
CDK4 Monoclonal Antibody (DCS-31.2)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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CDK4 Antibody (MA1-82085) in WB
Western blot was performed using Anti-CDK4 Monoclonal Antibody (DCS-31.2) (Product # MA1-82085) and a 33kDa band corresponding to CDK4 was observed across the cell lines tested and tissues tested along with uncharacterized band (*). Whole cell extracts (30 µg lysate) of K-562 (Lane 1), A549 (Lane 2), HeLa (Lane 3), NIH/3T3 (Lane 4), PC12 (Lane 5), Mouse Lung (Lane 6), Rat Lung (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane ... View More
产品信息
MA1-82085
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
DCS-31.2
抗原
Proprietary Immunogen.
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.08% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_929804
产品详细信息
Mouse anti CDK4 antibody clone DCS31.2 recognizes cyclin dependent kinase 4 (Cdk4), a ~34 kDa protein involved in the regulation of cell cycle progression between G1 and S.Mouse anti CDK4 antibody clone DCS31.2 does not cross react with other cdk's and does not co-precipitate the D type cyclins bound to cdk4.
靶标信息
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名:
Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; p34
基因别名: CDK4; CMM3; Crk3; PSK-J3
UniProt ID: (Human) P11802, (Rat) P35426, (Mouse) P30285
Entrez Gene ID: (Human) 1019, (Rat) 94201, (Mouse) 12567
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
cyclin-dependent protein serine/threonine kinase regulator activity
cyclin binding
protein complex binding
protein kinase activity
kinase activity
nucleotide binding
protein serine/threonine kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
lens development in camera-type eye
protein phosphorylation
signal transduction
circadian rhythm
positive regulation of cell proliferation
response to toxic substance
response to lead ion
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
organ regeneration
response to testosterone
response to drug
positive regulation of apoptotic process
positive regulation of translation
positive regulation of cell cycle
positive regulation of cell size
regulation of protein kinase activity
positive regulation of fibroblast proliferation
cell division
response to hyperoxia
negative regulation of cell cycle arrest
response to organic substance
regulation of cell proliferation
regulation of cell cycle
cell cycle
phosphorylation
