Invitrogen

CDK6 Polyclonal Antibody

查看其他24个CDK6抗体

抗体检测数据 (3)

CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CDK6 Antibody (PA5-79027) in IHC (P)

Immunohistochemistry analysis of CDK6 on paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with CDK6 polyclonal antibody (Product# PA5-79027) with a dilution of 2 µg/mL (overnight at 4°C), followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). De... View More

CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CDK6 Antibody in Western Blot (WB)

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CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CDK6 on paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with CDK6 polyclonal antibody (Product# PA5-79027) with a dilution of 2 µg/mL (overnight at 4°C), followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CDK6 on paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with CDK6 polyclonal antibody (Product# PA5-79027) with a dilution of 2 µg/mL (overnight at 4°C), followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

CDK6 Antibody in Western Blot (WB) — Western blot analysis of CDK6 in Lane 1: human HeLa whole cell lysate, Lane 2: human SGC-7901 whole cell lysate, Lane 3: human K562 whole cell lysate, Lane 4: mouse SP20 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with CDK6 polyclonal antibody (Product # PA5-79027) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

产品信息

PA5-79027

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human Cdk6 recombinant protein (Position: M1-A326).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2746143

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

CDK6 functions as a cell-cycle initiator protein. This protein is coexpressed and copurified with CyclinD1. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cell division protein kinase 6; CR2 protein kinase; CRK2; Cyclin-dependent kinase 6; MGC59692; Serine/threonine-protein kinase PLSTIRE

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基因别名: 5830411I20; AI504062; CDK6; CDKN6; Crk2; MCPH12; PLSTIRE

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UniProt ID: (Human) Q00534, (Mouse) Q64261

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Entrez Gene ID: (Human) 1021, (Rat) 114483, (Mouse) 12571

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cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding cyclin binding kinase activity nucleotide binding protein kinase activity protein serine/threonine kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

参与通路

G1/S transition of mitotic cell cycle positive regulation of cell-matrix adhesion type B pancreatic cell development protein phosphorylation cell cycle arrest Notch signaling pathway negative regulation of cell proliferation response to virus regulation of gene expression positive regulation of gene expression astrocyte development dentate gyrus development lateral ventricle development T cell differentiation in thymus gliogenesis cell dedifferentiation negative regulation of cell differentiation negative regulation of myeloid cell differentiation regulation of erythrocyte differentiation negative regulation of monocyte differentiation negative regulation of osteoblast differentiation negative regulation of cell cycle positive regulation of fibroblast proliferation generation of neurons negative regulation of epithelial cell proliferation cell division hematopoietic stem cell differentiation cellular response to phorbol 13-acetate 12-myristate regulation of cell motility negative regulation of cellular senescence hematopoietic progenitor cell differentiation phosphorylation hemopoiesis cell cycle cell differentiation regulation of cell proliferation