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CDK9 Antibody (PA5-87517) in IHC (P)
Immunohistochemistry analysis of CDK9 in paraffin-embedded human thyroid cancer. Samples were incubated with CDK9 Polyclonal antibody (Product # PA5-87517) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
产品信息
PA5-87517
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_0012521)
偶联物
形式
Liquid
浓度
1.6 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804217
产品详细信息
Immunogen sequence: ANVLITRDGV LKLADFGLAR AFSLAKNSQP NRYTNRVVTL WYRPPELLLG ERDYGPPIDL WGAGCIMAEM WTRSPIMQGN TEQHQLALIS QLCGSITPEV WPNVDNYELY EKLELVKGQK RKVKDRLKAY VRDPYALDLI DKLLVLDPAQ RIDSDDALNH DFFWSDPMPS DLKGMLSTHL TSMFEYLAPP RRKGSQITQQ STNQSRNPAT TNQTEFERVF; Positive Samples: LO2, HeLa, A-549, SKOV3, Mouse brain, Rat testis; Cellular Location: Cytoplasm, Nucleus, PML body
靶标信息
CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-2K; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit
基因别名: C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK
UniProt ID: (Human) P50750, (Mouse) Q99J95, (Rat) Q641Z4
Entrez Gene ID: (Human) 1025, (Mouse) 107951, (Rat) 362110
RNA polymerase II core promoter sequence-specific DNA binding
transcription coactivator binding
DNA binding
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
7SK snRNA binding
nucleotide binding
transcription factor binding
transferase activity
transferase activity, transferring phosphorus-containing groups
snRNA binding
transcription regulatory region DNA binding
non-receptor serine/threonine protein kinase
DNA repair
regulation of DNA repair
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein phosphorylation
regulation of mitotic cell cycle
cell proliferation
positive regulation of cardiac muscle hypertrophy
regulation of histone modification
replication fork processing
positive regulation of histone phosphorylation
response to drug
snRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
regulation of muscle cell differentiation
negative regulation of cell cycle arrest
cellular response to cytokine stimulus
negative regulation of mRNA polyadenylation
positive regulation of mRNA 3'-UTR binding
positive regulation of histone H2B ubiquitination
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
phosphorylation
replication fork arrest
