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CDKN2B Antibody (CF505205) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B (RC204895, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN2. Positive lysates LY417638 (100 µg) and LC417638 (20 µg) can be purchased separately from OriGene.
产品信息
CF505205
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
OTI7C4
抗原
Full length human recombinant protein of human CDKN2B produced in HEK293T cell.
偶联物
Unconjugated
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
The four known 15-to 19 kDa INK4 proteins (p15INK4b, p16INK4a, p18INK4c andp19INK4d) bind and inhibit CDK4 and CDK6 but not other CDKs, including cyclin E-Cdk2, cyclin A-Cdk2 and cyclin B-Cdk1. Like the three D-type cyclins, the INK4 genes are expressed in distinct tissue-specific patterns, suggesting that they are not strictly redundant. 5 p18INK4c is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CDK inhibitory protein; CDK4B inhibitor; Cyclin-dependent kinase 4 inhibitor B; cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); cyclin-dependent kinases 4 and 6 binding protein; MTS-2; Multiple tumor suppressor 2; p14-INK4b; p14_CDK inhibitor; p14_INK4B; p15 CDK inhibitor; p15-INK4b; p15_INK4B; p15INK4B
基因别名: CDK4I; CDKN2B; INK4B; MTS2; P15; p15INK4b; TP15
UniProt ID: (Human) P42772
Entrez Gene ID: (Human) 1030
regulation of cyclin-dependent protein serine/threonine kinase activity
G2/M transition of mitotic cell cycle
cell cycle arrest
mitotic cell cycle checkpoint
negative regulation of cell proliferation
megakaryocyte differentiation
positive regulation of transforming growth factor beta receptor signaling pathway
cellular response to extracellular stimulus
cellular response to nutrient
negative regulation of phosphorylation
positive regulation of transcription from RNA polymerase II promoter
spleen development
negative regulation of epithelial cell proliferation
negative regulation of protein serine/threonine kinase activity
negative regulation of G1/S transition of mitotic cell cycle
