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CENPA Antibody (PA5-75860) in WB
Western blot was performed using Anti-CENPA Polyclonal Antibody(Product # PA5-75860) and a 17kDa band corresponding to CENPA was observed across all the cell lines tested. Nuclear enriched extracts (30 µg lysate) of SW480 (Lane 1), HT-29 (Lane 2), Jurkat (Lane 3) and IMR32 (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primar... View More
产品信息
PA5-75860
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues in Human CENPA.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2719588
产品详细信息
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
靶标信息
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A
基因别名: CenH3; CENP-A; CENPA; RGD1563607
UniProt ID: (Human) P49450, (Mouse) O35216
Entrez Gene ID: (Human) 1058, (Mouse) 12615, (Rat) 298850
